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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1990-10-11
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pubmed:abstractText |
Activation of the c-abl protooncogene occurs in Abelson murine leukemia virus, in Hardy-Zuckerman 2 feline sarcoma virus, and during the chromosomal translocations that generate BCR-ABL gene fusion products. To study the molecular mechanism involved in the c-abl activation, we have created a series of modifications in murine c-abl and assayed these constructs for oncogenic activity using the NIH 3T3 cell transformation assay. Our results show that amino-terminal deletions are sufficient for oncogenic activation of c-abl and high levels of oncogenic activities were generated by a deletion of 114 codons from the 5' end that deleted the SH3 region. A deletion of 53 codons from the 5' end (inclusive of deletions seen in Hardy-Zuckerman 2 feline sarcoma virus and BCR-ABL gene products) that retains the SH3 region of c-abl resulted in the generation of low levels of transforming activity. This transforming potential was substantially increased with the introduction of a G----A point mutation in codon 832 that is present in v-abl. The point mutation was found to affect the secondary structure and the tyrosine kinase activity of the mutant gene products.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-163444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2405279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2432397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2440107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2465527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2496972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2542016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2554240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2670246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2687768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2836147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2852893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2982232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-2989692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-3029415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-3047582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-3078956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-3317402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-3498165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-364941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-3879812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6204766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6244493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6257398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6304726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6306469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6316147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168550-6330085
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6502-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2168550-Abelson murine leukemia virus,
pubmed-meshheading:2168550-Animals,
pubmed-meshheading:2168550-Cell Line,
pubmed-meshheading:2168550-Cell Transformation, Neoplastic,
pubmed-meshheading:2168550-Chromosome Deletion,
pubmed-meshheading:2168550-Gene Expression Regulation,
pubmed-meshheading:2168550-Genetic Vectors,
pubmed-meshheading:2168550-Leukemia Virus, Murine,
pubmed-meshheading:2168550-Mice,
pubmed-meshheading:2168550-Mutation,
pubmed-meshheading:2168550-Plasmids,
pubmed-meshheading:2168550-Protein-Tyrosine Kinases,
pubmed-meshheading:2168550-Proto-Oncogene Proteins,
pubmed-meshheading:2168550-Proto-Oncogene Proteins c-abl,
pubmed-meshheading:2168550-Proto-Oncogenes,
pubmed-meshheading:2168550-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Activation of murine c-abl protooncogene: effect of a point mutation on oncogenic activation.
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pubmed:affiliation |
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104-4268.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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