Source:http://linkedlifedata.com/resource/pubmed/id/21683789
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
2011-7-6
|
pubmed:abstractText |
Cancer cell invasion plays a crucial role in growth and local spreading of tumors. GL-V9 is a newly synthesized flavonoid that has been shown to possess an antitumor effect. However, the mechanism of GL-V9 in preventing tumor growth is still unclear. The purpose of this study is to investigate the anti-invasive and anti-metastatic activity of this novel compound in MDA-MB-231 and MCF-7 human breast carcinoma cells. In this study, GL-V9 caused a concentration-dependent suppression of cell adhesive ability by cell adhesion assay, it also inhibited the migration and invasion of cells by wound healing assay and transwell invasion assay in a concentration-dependent manner. Considering matrix metalloproteinases (MMPs) play an important role in metastatic process, we used western blotting and gelatin zymography to examine the effect of GL-V9 on the expression and activity of MMPs. The mechanism revealed that GL-V9 significantly suppressed the expression and activity of MMP-2 and MMP-9. Furthermore, GL-V9 suppressed their upstream protein kinases activation by reducing phosphorylated forms of serine/threonine kinase AKT and c-Jun N-terminal kinase. These findings suggested that GL-V9 could inhibit the invasion of tumor cells by downregulating the expression and activity of MMP-2 and MMP-9, potentially associating with the suppression of phosphorylation of AKT and JNK.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
1879-0720
|
pubmed:author | |
pubmed:copyrightInfo |
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
|
pubmed:issnType |
Electronic
|
pubmed:day |
17
|
pubmed:volume |
43
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
393-9
|
pubmed:meshHeading |
pubmed-meshheading:21683789-Blotting, Western,
pubmed-meshheading:21683789-Breast Neoplasms,
pubmed-meshheading:21683789-Cell Adhesion,
pubmed-meshheading:21683789-Cell Line, Tumor,
pubmed-meshheading:21683789-Down-Regulation,
pubmed-meshheading:21683789-Female,
pubmed-meshheading:21683789-Flavonoids,
pubmed-meshheading:21683789-Humans,
pubmed-meshheading:21683789-Matrix Metalloproteinase 2,
pubmed-meshheading:21683789-Matrix Metalloproteinase 9,
pubmed-meshheading:21683789-Neoplasm Invasiveness,
pubmed-meshheading:21683789-Phosphorylation
|
pubmed:year |
2011
|
pubmed:articleTitle |
Inhibitory effects of GL-V9 on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix metalloproteinase-2/9.
|
pubmed:affiliation |
Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Tongjiaxiang, Nanjing, People's Republic of China.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|