Linked Life Data

2168112

Source:http://linkedlifedata.com/resource/pubmed/id/2168112

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-10-2
pubmed:abstractText
The nucleoprotein organization of herpes simplex virus type 1 (HSV-1) DNA during productive infection was analyzed using staphylococcal nuclease. Both prior to and during the genome replication phase of infection, digestion of nuclei revealed two readily discernible forms of viral DNA, resistant and sensitive. The identity of these forms was established by the use of a variety of assays, including velocity sedimentation, nucleic acid hybridization and restriction endonuclease digestion and by employing temperature sensitive (ts) mutants impaired in either DNA replication or encapsidation of progeny DNA. Thus, nuclease resistant DNA was derived from encapsidated unit length genomes while sensitive DNA represented digestion products of replicating viral genomes. Importantly, no evidence was obtained for the arrangement of either parental or progeny viral DNA in nucleosomes. These findings are discussed with regard to the nucleoprotein structure of replicating viral DNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Intracellular organization of herpes simplex virus type 1 DNA assayed by staphylococcal nuclease sensitivity.
pubmed:affiliation
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27514.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.