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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1990-10-4
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pubmed:databankReference |
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pubmed:abstractText |
The genes which encode glycosomal glyceraldehyde-phosphate dehydrogenase (gGAPDH) of Trypanosoma cruzi are arranged as a tandemly repeated pair on a single chromosome and are identical at the level of nucleotide sequence. They are separated by an intergenic region which contains a 317 base pair sequence with the properties of a retroposon. The genes express a 1.5 kb mRNA and a 38 kd protein. The amino acid sequence contains features characteristic of glycosomal enzymes such as peptide insertions and a C-terminal extension. However, T. cruzi gGAPDH lacks one of the positively charged 'hotspot' motifs which have been proposed as topogenic signals for import into the glycosome, a unique microbody-like organelle. Molecular modelling of the T. cruzi and T. brucei enzymes suggests that neither structure would fulfil the requirements of the 'hotspot' glycosomal import model.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-15462837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2300209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2304458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2427017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2455228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2483088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2499047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2654139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2655706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2676581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2792108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2831499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2835672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2841110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2874767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2972929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2977087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-2987855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3013612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3015599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3076722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3104618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3120638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3191913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3221866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3267227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3276517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3320052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3386689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3402434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3508286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3508287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3516224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3525150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3550435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3554143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3582360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3586018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3595556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3608983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-3858856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-4294736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-6327058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2167831-6502707
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2751-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2167831-Amino Acid Sequence,
pubmed-meshheading:2167831-Animals,
pubmed-meshheading:2167831-Base Sequence,
pubmed-meshheading:2167831-Chromosome Mapping,
pubmed-meshheading:2167831-DNA Transposable Elements,
pubmed-meshheading:2167831-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:2167831-Introns,
pubmed-meshheading:2167831-Models, Molecular,
pubmed-meshheading:2167831-Molecular Sequence Data,
pubmed-meshheading:2167831-Multigene Family,
pubmed-meshheading:2167831-Organelles,
pubmed-meshheading:2167831-Protein Conformation,
pubmed-meshheading:2167831-Restriction Mapping,
pubmed-meshheading:2167831-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2167831-Signal Transduction,
pubmed-meshheading:2167831-Trypanosoma cruzi
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pubmed:year |
1990
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pubmed:articleTitle |
Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase does not conform to the 'hotspot' topogenic signal model.
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pubmed:affiliation |
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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