|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2011-7-27
|
|
pubmed:abstractText |
The transcription factor Bcl6 regulates germinal center formation and differentiation of B cells into high-affinity antibody-producing plasma cells. The direct double-negative regulatory circuit between Bcl6 and Blimp-1 is well established. We now reveal alternative mechanisms for Bcl6-mediated regulation of B-cell differentiation to plasma cells and show with DT40 cells that Bcl6 directly promotes the expression of Bach2, a known suppressor of Blimp-1. Moreover, Bcl6 suppresses Blimp-1 expression through direct binding to the IRF4 gene, as well as by promoting the expression of MITF, a known suppressor of IRF4. We also provide evidence that Bcl6 is needed for the expression of AID and UNG, the indispensable proteins for somatic hypermutation and class-switch recombination, and UNG appears to be a direct Bcl6 target. Our findings reveal a complex regulatory network in which Bcl6 acts as a key element dictating the transition of DT40 B cells to plasma cells.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1521-4141
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
41
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2404-13
|
|
pubmed:meshHeading |
pubmed-meshheading:21674482-Animals,
pubmed-meshheading:21674482-Avian Proteins,
pubmed-meshheading:21674482-B-Cell-Specific Activator Protein,
pubmed-meshheading:21674482-B-Lymphocytes,
pubmed-meshheading:21674482-Basic-Leucine Zipper Transcription Factors,
pubmed-meshheading:21674482-Blotting, Western,
pubmed-meshheading:21674482-Cell Differentiation,
pubmed-meshheading:21674482-Cell Line, Tumor,
pubmed-meshheading:21674482-Chickens,
pubmed-meshheading:21674482-Gene Expression Profiling,
pubmed-meshheading:21674482-Interferon Regulatory Factors,
pubmed-meshheading:21674482-Models, Immunological,
pubmed-meshheading:21674482-Mutation,
pubmed-meshheading:21674482-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:21674482-Plasma Cells,
pubmed-meshheading:21674482-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21674482-Signal Transduction,
pubmed-meshheading:21674482-Transcription Factors
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Alternate pathways for Bcl6-mediated regulation of B cell to plasma cell differentiation.
|
|
pubmed:affiliation |
Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland. jukka.alinikula@utu.fi
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
