Linked Life Data

21668366

Source:http://linkedlifedata.com/resource/pubmed/id/21668366

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-6-14
pubmed:abstractText
HIV infections are mainly acquired by mucosal transmission, through oral, rectal, or genital mucosa. Epithelial cells (EC) are the first cells encountered by HIV during infection through sexual transmission and breastfeeding. EC express several receptors critical for both primary HIV infection and secondary transmission. The regulation of co-receptor expression correlates with changes in susceptibility to infection by HIV-1 strains with different tropism. Moreover, inflammatory responses at mucosal surfaces after HIV-1 transmission may influence disease outcome. In the present study, we analyzed the effect of the accessory HIV-1 Nef protein on mucosal EC, using unstimulated or IFN-?-stimulated HEp-2, T24, and Caco2?cell lines as models for homeostatic or inflamed mucosal tracts. We found that Nef significantly upregulated the expression of CXCR4 on the Caco-2?cell surface and the expression of galactosylceramide on the T24?cell surface. In addition, Nef significantly upregulated IL-6 production by T24 and Caco-2?cells, and TNF-? release by all three cell lines analyzed. Notably, Nef abrogated the IFN-?-induced modulation of co-receptor expression and cytokine secretion. Our findings suggest that Nef differently regulates co-receptor expression and cytokine secretion at the epithelial level, depending on the anatomical derivation of the cells and the inflammatory status.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1557-8976
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-50
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Effects of HIV-1 Nef on virus co-receptor expression and cytokine release in human bladder, laryngeal, and intestinal epithelial cell lines.
pubmed:affiliation
Department of Therapeutic Research and Medicine Evaluation, Istituto Superiore di Sanità, Roma, Italy. mariagiovanna.quaranta@iss.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't