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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2011-7-5
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pubmed:abstractText |
The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1791-244X
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pubmed:author |
pubmed-author:AmistàPietroP,
pubmed-author:BaigueraSilviaS,
pubmed-author:BiancoAlessandraA,
pubmed-author:ConconiMaria TeresaMT,
pubmed-author:DalzoppoDanieleD,
pubmed-author:Del GaudioCostantinoC,
pubmed-author:Di LiddoRosaR,
pubmed-author:GrandiClaudioC,
pubmed-author:LoraSilvanoS,
pubmed-author:MartorinaFrancescaF,
pubmed-author:ParnigottoPier PaoloPP
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pubmed:issnType |
Electronic
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
315-25
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pubmed:meshHeading |
pubmed-meshheading:21667016-Animals,
pubmed-meshheading:21667016-Blood Vessel Prosthesis,
pubmed-meshheading:21667016-Blood Vessels,
pubmed-meshheading:21667016-Cattle,
pubmed-meshheading:21667016-Cell Adhesion,
pubmed-meshheading:21667016-Cell Proliferation,
pubmed-meshheading:21667016-Cells, Cultured,
pubmed-meshheading:21667016-Collagen,
pubmed-meshheading:21667016-Cross-Linking Reagents,
pubmed-meshheading:21667016-Endothelial Cells,
pubmed-meshheading:21667016-Epoxy Resins,
pubmed-meshheading:21667016-Glycine,
pubmed-meshheading:21667016-Humans,
pubmed-meshheading:21667016-Immunohistochemistry,
pubmed-meshheading:21667016-Microscopy, Electron, Scanning,
pubmed-meshheading:21667016-Myocytes, Smooth Muscle,
pubmed-meshheading:21667016-Tissue Engineering,
pubmed-meshheading:21667016-Trypsin
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pubmed:year |
2011
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pubmed:articleTitle |
Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts.
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pubmed:affiliation |
Department of Pharmaceutical Sciences, University of Padua, Via Marzolo 5, I-35131 Padua, Italy. claudio.grandi@unipd.it
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pubmed:publicationType |
Journal Article
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