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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0043457,
umls-concept:C0185117,
umls-concept:C0439855,
umls-concept:C0486616,
umls-concept:C0851285,
umls-concept:C1336596,
umls-concept:C1336789,
umls-concept:C1413302,
umls-concept:C1709059,
umls-concept:C2911684
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pubmed:issue |
14
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pubmed:dateCreated |
2011-7-20
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pubmed:abstractText |
The caudal homeobox (cdx) gene family is critical for specification of caudal body formation and erythropoiesis. In zebrafish, cdx4 expression is controlled by the Wnt pathway, but the molecular mechanism of this regulation is not fully understood. Here, we provide evidence that Tcf3 suppresses cdx4 expression through direct binding to multiple sites in the cdx4 gene regulatory region. Tcf3 requires corepressor molecules such as Groucho (Gro)/TLE and HDAC1 for activity. Using zebrafish embryos and cultured mammalian cells, we show that the transcription factor E4f1 derepresses cdx4 by dissociating corepressor proteins from Tcf3 without inhibiting its binding to cis-regulatory sites in the DNA. Further, the E3 ubiquitin ligase Lnx2b, acting as a scaffold protein irrespective of its enzymatic activity, counteracts the effects of E4f1. We propose that the modulation of Tcf3 repressor function by E4f1 assures precise and robust regulation of cdx4 expression in the caudal domain of the embryo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/HDAC1 protein, zebrafish,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/cdx4 protein, zebrafish,
http://linkedlifedata.com/resource/pubmed/chemical/gro3 protein, zebrafish
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pubmed:status |
MEDLINE
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pubmed:issn |
1460-2075
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2894-907
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pubmed:meshHeading |
pubmed-meshheading:21666599-Animals,
pubmed-meshheading:21666599-Blotting, Western,
pubmed-meshheading:21666599-Body Patterning,
pubmed-meshheading:21666599-Cells, Cultured,
pubmed-meshheading:21666599-Chromatin Immunoprecipitation,
pubmed-meshheading:21666599-Embryo, Nonmammalian,
pubmed-meshheading:21666599-Gene Expression Regulation, Developmental,
pubmed-meshheading:21666599-Histone Deacetylase 1,
pubmed-meshheading:21666599-Homeodomain Proteins,
pubmed-meshheading:21666599-Immunoprecipitation,
pubmed-meshheading:21666599-Luciferases,
pubmed-meshheading:21666599-Promoter Regions, Genetic,
pubmed-meshheading:21666599-Protein Binding,
pubmed-meshheading:21666599-RNA, Messenger,
pubmed-meshheading:21666599-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:21666599-Repressor Proteins,
pubmed-meshheading:21666599-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21666599-Ubiquitin-Protein Ligases,
pubmed-meshheading:21666599-Wnt Proteins,
pubmed-meshheading:21666599-Zebrafish,
pubmed-meshheading:21666599-Zebrafish Proteins
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pubmed:year |
2011
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pubmed:articleTitle |
Modulation of Tcf3 repressor complex composition regulates cdx4 expression in zebrafish.
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pubmed:affiliation |
Laboratory of Molecular Genetics, Program in Genomics of Differentiation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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