Source:http://linkedlifedata.com/resource/pubmed/id/21665965
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
16
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pubmed:dateCreated |
2011-7-29
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pubmed:abstractText |
HAMP domains are sensory transduction modules that connect input and output domains in diverse signaling proteins from archaea, bacteria, and lower eukaryotes. Here, we employed in vivo disulfide cross-linking to explore the structure of the HAMP domain in the Escherichia coli aerotaxis receptor Aer. Using an Aer HAMP model based on the structure of Archaeoglobus fulgidus Af1503-HAMP, the closest residue pairs at the interface of the HAMP AS-1 and AS-2' helices were determined and then replaced with cysteines and cross-linked in vivo. Except for a unique discontinuity in AS-2, the data suggest that the Aer HAMP domain forms a parallel four-helix bundle that is similar to the structure of Af1503. The HAMP discontinuity was associated with a segment of AS-2 that was recently shown to interact with the Aer-PAS sensing domain. The four-helix HAMP bundle and its discontinuity were maintained in both the kinase-on and kinase-off states of Aer, although differences in the rates of disulfide formation also indicated the existence of different HAMP conformations in the kinase-on and kinase-off states. In particular, the kinase-on state was accompanied by significantly increased disulfide formation rates at the distal end of the HAMP four-helix bundle. This indicates that HAMP signaling may be associated with a tilting of the AS-1 and AS-2' helices, which may be the signal that is transmitted to the kinase control region of Aer.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1098-5530
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4095-103
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pubmed:meshHeading |
pubmed-meshheading:21665965-Archaeal Proteins,
pubmed-meshheading:21665965-Archaeoglobus fulgidus,
pubmed-meshheading:21665965-Gene Expression Regulation, Archaeal,
pubmed-meshheading:21665965-Models, Molecular,
pubmed-meshheading:21665965-Mutagenesis, Site-Directed,
pubmed-meshheading:21665965-Protein Conformation,
pubmed-meshheading:21665965-Protein Structure, Tertiary,
pubmed-meshheading:21665965-Signal Transduction
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pubmed:year |
2011
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pubmed:articleTitle |
Different conformations of the kinase-on and kinase-off signaling states in the Aer HAMP domain.
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pubmed:affiliation |
Division of Microbiology and Molecular Genetics, Loma Linda University, Loma Linda, CA 92350, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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