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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1990-9-12
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pubmed:abstractText |
The tegument of human cytomegalovirus (HCMV) contains a phosphorylated protein of 65 kilodaltons, termed pp65, which was reported to carry significant epitopes for the stimulation of the humoral immune response during natural infection. A monoclonal antibody directed against this protein was used to screen a lambda gt11 cDNA library for recombinant polypeptides. Two DNA fragments from purified lambda clones and one fragment from genomic DNA were used for cloning in a bacterial high-level expression vector. The resulting fusion proteins were tested for their reactivity with a panel of monoclonal antibodies directed against pp65 and with polyspecific anti-HCMV rabbit antisera. The binding site for all the monoclonal antibodies tested was found to be contained in one of the recombinant proteins with a viral portion of 26 amino acids. Immunoblot analyses with HCMV-positive human sera revealed that pp65 alone is not a reliable antigen for serodiagnosis but may be very useful in combination with other HCMV proteins.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2455019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2536831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2536837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2539706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2546972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2549097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2555378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2580024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2826612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2836608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2838967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2839611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2841381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2848898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2851606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2852670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2873201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2898044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2901164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2985644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2993668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2997607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-2997930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3001220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3002068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3005437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3005516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3005633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3016333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3027374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3033138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3037013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-3040869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6087357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6284646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6295952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6310864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6317893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6322416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6324477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166078-6329369
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1229-35
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2166078-Animals,
pubmed-meshheading:2166078-Antibodies, Monoclonal,
pubmed-meshheading:2166078-Antibodies, Viral,
pubmed-meshheading:2166078-Blotting, Western,
pubmed-meshheading:2166078-Cells, Cultured,
pubmed-meshheading:2166078-Cytomegalovirus,
pubmed-meshheading:2166078-DNA, Recombinant,
pubmed-meshheading:2166078-Gene Expression,
pubmed-meshheading:2166078-Humans,
pubmed-meshheading:2166078-Phosphoproteins,
pubmed-meshheading:2166078-Rabbits,
pubmed-meshheading:2166078-Recombinant Proteins,
pubmed-meshheading:2166078-Restriction Mapping,
pubmed-meshheading:2166078-Viral Envelope Proteins,
pubmed-meshheading:2166078-Viral Proteins
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pubmed:year |
1990
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pubmed:articleTitle |
Procaryotic expression of phosphorylated tegument protein pp65 of human cytomegalovirus and application of recombinant peptides for immunoblot analyses.
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pubmed:affiliation |
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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