| pubmed-article:2165910 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2165910 | lifeskim:mentions | umls-concept:C0085979 | lld:lifeskim |
| pubmed-article:2165910 | lifeskim:mentions | umls-concept:C0025166 | lld:lifeskim |
| pubmed-article:2165910 | lifeskim:mentions | umls-concept:C0162745 | lld:lifeskim |
| pubmed-article:2165910 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:2165910 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:2165910 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:2165910 | pubmed:dateCreated | 1990-9-7 | lld:pubmed |
| pubmed-article:2165910 | pubmed:abstractText | Cultured guinea pig bone marrow megakaryocytes were found to secrete a 92-kd collagenase that was detected by digestion of gelatin in a polyacrylamide substrate gel assay. Neither casein or bovine serum albumin were digested by this enzyme. The enzyme is a neutral metalloprotease. Its secretion is increased by thrombin (1.0 U/ml) and phorbol myristate acetate (10 ng/ml) and is unaffected by prostaglandin E1 (10 microM). In the absence of serum, gelatinase secretion is inhibited, but it can be stimulated by cytochalasin D (1.0 microgram/ml). Gelatinase activity in the medium from megakaryocytes cultured on rat tail collagen gel is decreased. Medium from megakaryocytes cultured on Matrigel contains a second gelatinase of 90 kd. Addition of the tetrapeptide RGDS to the cultures on Matrigel blocks the appearance of the 90-kd gelatinase. Platelets contained both a 92- and a 90-kd gelatinase that was detected only after thrombin activation. The results indicate that megakaryocytes can secrete a collagenase, and its secretion may be in part controlled by interaction with the extracellular matrix. The appearance of the 90-kd gelatinase may be associated with megakaryocyte maturation and platelet formation. | lld:pubmed |
| pubmed-article:2165910 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2165910 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2165910 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2165910 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2165910 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2165910 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2165910 | pubmed:issn | 0301-472X | lld:pubmed |
| pubmed-article:2165910 | pubmed:author | pubmed-author:LevenR MRM | lld:pubmed |
| pubmed-article:2165910 | pubmed:author | pubmed-author:YeeTT | lld:pubmed |
| pubmed-article:2165910 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2165910 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:2165910 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2165910 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2165910 | pubmed:pagination | 743-7 | lld:pubmed |
| pubmed-article:2165910 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:meshHeading | pubmed-meshheading:2165910-... | lld:pubmed |
| pubmed-article:2165910 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2165910 | pubmed:articleTitle | Collagenase production by guinea pig megakaryocytes in vitro. | lld:pubmed |
| pubmed-article:2165910 | pubmed:affiliation | Research Medicine and Radiation Biophysics Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720. | lld:pubmed |
| pubmed-article:2165910 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2165910 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2165910 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2165910 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
