Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1990-8-31
pubmed:abstractText
Mutations in genes not required for exponential growth but essential for survival in stationary phase were isolated in an effort to understand the ability of wild-type Escherichia coli cells to remain viable during prolonged periods of nutritional deprivation. The phenotype of these mutations is referred to as Sur- (survival) and the genes are designated sur. The detailed analysis of one of these mutations is presented here. The mutation (surA1) caused by insertion of a mini-Tn10 element defined a new gene located near 1 min on the E. coli chromosome. It was located directly upstream of pdxA and formed part of a complex operon. Evidence is presented supporting the interpretation that cells harboring the surA1 mutation die during stationary phase while similar insertion mutations in other genes of the operon do not lead to a Sur- phenotype. Strains harboring surA1 had a normal doubling time in both rich and minimal medium, but cultures lost viability after several days in stationary phase. Analysis of revertants and suppressors of surA1, which arose after prolonged incubation in stationary phase, indicates that DNA rearrangements (excisions and duplications) occurred in cultures of this strain even when the viable-cell counts were below 10(2) cells per ml. Cells containing suppressing mutations then grew in the same culture to 10(8) cells per ml, taking over the population. The implications of these observations to our understanding of stationary-phase mutagenesis are discussed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-13975747, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2407962, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2422153, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2478072, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2537825, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2544886, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2651423, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2670894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2684651, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2690013, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2740338, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2830029, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2835580, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2852143, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-2982787, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3017916, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3031429, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3038334, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3045565, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3275619, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3312987, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3517593, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3536847, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3884591, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-3904630, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-4162516, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-4966830, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6099322, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6233472, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6256606, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6263926, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6282821, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6323254, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6389505, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-6450312, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-7005624, http://linkedlifedata.com/resource/pubmed/commentcorrection/2165476-762018
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4339-47
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
surA, an Escherichia coli gene essential for survival in stationary phase.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't