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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1990-8-24
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pubmed:abstractText |
In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71 ( Pt 7)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1591-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2165138-Animals,
pubmed-meshheading:2165138-Base Sequence,
pubmed-meshheading:2165138-Cell Line,
pubmed-meshheading:2165138-Chromosome Deletion,
pubmed-meshheading:2165138-DNA, Viral,
pubmed-meshheading:2165138-Genes, Viral,
pubmed-meshheading:2165138-Herpesvirus 1, Suid,
pubmed-meshheading:2165138-Molecular Sequence Data,
pubmed-meshheading:2165138-Mutation,
pubmed-meshheading:2165138-Oligonucleotide Probes,
pubmed-meshheading:2165138-Restriction Mapping,
pubmed-meshheading:2165138-Swine,
pubmed-meshheading:2165138-Thymidine Kinase,
pubmed-meshheading:2165138-Viral Structural Proteins,
pubmed-meshheading:2165138-Viral Vaccines
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pubmed:year |
1990
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pubmed:articleTitle |
Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain.
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pubmed:affiliation |
Department of Virology, Central Veterinary Institute (CDI), Lelystad, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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