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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1990-8-27
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pubmed:abstractText |
The epidemiology of rotavirus gastroenteritis was investigated for two consecutive seasons (1987-1988 and 1988-1989) in seven locales in the continental USA. The 281 representative fecal samples obtained from children with diarrhea were electropherotyped and serotyped by an enzyme immunoassay with serotype-specific monoclonal antibodies and a new amplification typing technique (polymerase chain reaction typing). Serotype 1 was predominant in both years, particularly in the North and East; serotype 3 was second in frequency and found most often in the South; serotype 2 was detected only occasionally; serotypes 4, 8, and 9 were never found. Rotavirus strains were grouped into five major electropherotypes, each corresponded to a single serotype, and the relative migration of the gene segments 7-9 could be used to distinguish serotype 1 from serotype 3. The amplification typing technique proved to be of great value in typing the 17% of rotavirus-positive specimens untypable by the serologic technique.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1899
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
362-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2165108-Antibodies, Monoclonal,
pubmed-meshheading:2165108-Child,
pubmed-meshheading:2165108-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2165108-Feces,
pubmed-meshheading:2165108-Gastroenteritis,
pubmed-meshheading:2165108-Humans,
pubmed-meshheading:2165108-Immunoenzyme Techniques,
pubmed-meshheading:2165108-Polymerase Chain Reaction,
pubmed-meshheading:2165108-RNA, Viral,
pubmed-meshheading:2165108-Rotavirus,
pubmed-meshheading:2165108-Rotavirus Infections,
pubmed-meshheading:2165108-Seasons,
pubmed-meshheading:2165108-Serotyping,
pubmed-meshheading:2165108-United States
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pubmed:year |
1990
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pubmed:articleTitle |
Serotypes and electropherotypes of human rotavirus in the USA: 1987-1989.
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pubmed:affiliation |
Viral Gastroenteritis Unit, CDC, Atlanta, GA 30333.
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pubmed:publicationType |
Journal Article
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