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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2011-6-28
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pubmed:databankReference |
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pubmed:abstractText |
UNC119 is widely expressed among vertebrates and other phyla. We found that UNC119 recognized the acylated N terminus of the rod photoreceptor transducin ? (T?) subunit and Caenorhabditis elegans G proteins ODR-3 and GPA-13. The crystal structure of human UNC119 at 1.95-Å resolution revealed an immunoglobulin-like ?-sandwich fold. Pulldowns and isothermal titration calorimetry revealed a tight interaction between UNC119 and acylated G? peptides. The structure of co-crystals of UNC119 with an acylated T? N-terminal peptide at 2.0 Å revealed that the lipid chain is buried deeply into UNC119's hydrophobic cavity. UNC119 bound T?-GTP, inhibiting its GTPase activity, thereby providing a stable UNC119-T?-GTP complex capable of diffusing from the inner segment back to the outer segment after light-induced translocation. UNC119 deletion in both mouse and C. elegans led to G protein mislocalization. Thus, UNC119 is a G? subunit cofactor essential for G protein trafficking in sensory cilia.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP Phosphohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Gnat1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transducin,
http://linkedlifedata.com/resource/pubmed/chemical/UNC119 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/odr-3 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1546-1726
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pubmed:author |
pubmed-author:BaehrWolfgangW,
pubmed-author:ChenYangY,
pubmed-author:ConstantineRyanR,
pubmed-author:DavisM WayneMW,
pubmed-author:GerstnerCecilia DCD,
pubmed-author:HillChristopher PCP,
pubmed-author:HuangYuanpeng JanetYJ,
pubmed-author:InanaGeorgeG,
pubmed-author:JayaramanSeetharamanS,
pubmed-author:JorgensenErik MEM,
pubmed-author:MontelioneGaetano TGT,
pubmed-author:RongXiaoX,
pubmed-author:TongLiangL,
pubmed-author:VorobievSergeyS,
pubmed-author:WhitbyFrank GFG,
pubmed-author:ZhangHoubinH
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pubmed:issnType |
Electronic
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
874-80
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pubmed:dateRevised |
2011-9-30
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pubmed:meshHeading |
pubmed-meshheading:21642972-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:21642972-Animals,
pubmed-meshheading:21642972-Animals, Genetically Modified,
pubmed-meshheading:21642972-Caenorhabditis elegans,
pubmed-meshheading:21642972-Caenorhabditis elegans Proteins,
pubmed-meshheading:21642972-Cattle,
pubmed-meshheading:21642972-Dark Adaptation,
pubmed-meshheading:21642972-GTP Phosphohydrolases,
pubmed-meshheading:21642972-GTP-Binding Protein alpha Subunits,
pubmed-meshheading:21642972-GTP-Binding Protein alpha Subunits, G12-G13,
pubmed-meshheading:21642972-GTP-Binding Protein alpha Subunits, Gi-Go,
pubmed-meshheading:21642972-Gene Expression Regulation,
pubmed-meshheading:21642972-Glycine,
pubmed-meshheading:21642972-Green Fluorescent Proteins,
pubmed-meshheading:21642972-Humans,
pubmed-meshheading:21642972-Mice,
pubmed-meshheading:21642972-Mice, Knockout,
pubmed-meshheading:21642972-Models, Chemical,
pubmed-meshheading:21642972-Models, Molecular,
pubmed-meshheading:21642972-Mutation,
pubmed-meshheading:21642972-Protein Binding,
pubmed-meshheading:21642972-Protein Structure, Quaternary,
pubmed-meshheading:21642972-Protein Transport,
pubmed-meshheading:21642972-Sensory Receptor Cells,
pubmed-meshheading:21642972-Signal Transduction,
pubmed-meshheading:21642972-Time Factors,
pubmed-meshheading:21642972-Transducin
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pubmed:year |
2011
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pubmed:articleTitle |
UNC119 is required for G protein trafficking in sensory neurons.
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pubmed:affiliation |
Department of Ophthalmology, University of Utah Health Science Center, Salt Lake City, Utah, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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