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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-8-6
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pubmed:abstractText |
Disulphide bonds in human recombinant tissue inhibitor of metalloproteinases (TIMP) were assigned by resolving proteolytic digests of TIMP on reverse-phase h.p.l.c. and sequencing those peaks judged to contain disulphide bonds by virtue of a change in retention time on reduction. This procedure allowed the direct assignment of Cys-145-Cys-166 and the isolation of two other peptides containing two disulphide bonds each. Further peptide cleavage in conjunction with fast-atom-bombardment m.s. analysis permitted the assignments Cys-1-Cys-70, Cys-3-Cys-99, Cys-13-Cys-124 and Cys-127-Cys-174 from these peptides. The sixth bond Cys-132-Cys-137 was assigned by inference, as the native protein has no detectable free thiol groups.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-1001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-2793861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-2821028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-2982848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-3005266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-3162216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-3886013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-3894382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-3903517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-3919720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-558524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6094519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6272744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6272745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6313647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6321174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6370238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6661227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2163605-6906234
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
267-74
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2163605-Amino Acid Sequence,
pubmed-meshheading:2163605-Animals,
pubmed-meshheading:2163605-Cell Line,
pubmed-meshheading:2163605-Chromatography, High Pressure Liquid,
pubmed-meshheading:2163605-DNA,
pubmed-meshheading:2163605-Disulfides,
pubmed-meshheading:2163605-Glycoproteins,
pubmed-meshheading:2163605-Humans,
pubmed-meshheading:2163605-Hydrolysis,
pubmed-meshheading:2163605-Metalloendopeptidases,
pubmed-meshheading:2163605-Mice,
pubmed-meshheading:2163605-Microbial Collagenase,
pubmed-meshheading:2163605-Molecular Sequence Data,
pubmed-meshheading:2163605-Pepsin A,
pubmed-meshheading:2163605-Recombinant Proteins,
pubmed-meshheading:2163605-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:2163605-Trypsin
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pubmed:year |
1990
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pubmed:articleTitle |
Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP).
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pubmed:affiliation |
Biological Laboratory, University of Kent, Canterbury, U.K.
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pubmed:publicationType |
Journal Article
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