Linked Life Data

21635850

Source:http://linkedlifedata.com/resource/pubmed/id/21635850

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-6-3
pubmed:abstractText
The protein components of living cells in the hair follicle are amenable to study by standard molecular biological techniques, but identifying those in the hair shaft has been problematic until recently. Most of the protein, primarily keratins and keratin associated proteins, can be extracted under denaturing conditions, but 15-20% is intractable due to transglutaminase-mediated cross-linking. Shotgun proteomics now permits identifying >300 constituents of the isopeptide cross-linked proteome and even certain post-translational modifications. The proteins originate from all the intracellular compartments, indicating that the cross-linking process makes effective use of available resources to produce structures with great mechanical stability. Knowing this proteome provides a foundation for correlating defects in hair shaft structure with protein deficiencies. Such investigations can be extended to mouse models of aberrant pelage hair. Thus, inbred mouse strains can be distinguished by their hair proteomes, raising the possibility of similar variation in the human population. The nail plate is also amenable to this shotgun proteomic approach. Providing discrete and noninvasive sampling of the human proteome, these epidermal appendages could have diagnostic utility for certain disease states.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1525-7886
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-36
pubmed:meshHeading
pubmed:articleTitle
Proteomic analysis of hair shaft and nail plate.
pubmed:affiliation
Department of Environmental Toxicology, University of California, Davis, CA 95616-8588, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural