Linked Life Data

21635228

Source:http://linkedlifedata.com/resource/pubmed/id/21635228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-7-5
pubmed:abstractText
Studies have indicated that interleukin (IL)-10 has a pathogenic role in systemic lupus erythematosus (SLE); however, a protective effect of IL-10 in SLE was also observed. Because the exact mechanism of IL-10 signalling in the pathogenesis of SLE is unclear, this study sought to assess the expression and signalling of interleukin-10 receptor (IL-10R) in peripheral leucocytes from patients with SLE. We used flow cytometry to examine the expression of IL-10R1 on different peripheral leucocytes from 28 SLE patients, of whom 14 had lupus nephritis (LN) and 14 were healthy controls. We also examined the effects of IL-10 on phosphorylation of signal transducer and activator of transcription (STAT)-3 and STAT-1 in peripheral blood mononuclear cells (PBMCs) obtained from 13 SLE patients and seven healthy controls. Plasma cytokines were detected by flow cytometric bead array (CBA) techniques. Although IL-10R1 expression levels on each peripheral leucocyte subset from 28 SLE patients and 14 healthy controls were similar, the expression levels on CD4(+) T cells from LN patients were significantly lower than on CD4(+) T cells from controls and SLE patients without nephritis (P < 0·01). IL-10R1 expression levels on CD4(+) and CD8(+) T cells were correlated negatively with the SLE disease activity index (P < 0·01). Additionally, the phosphorylation of STAT-3 was delayed and reduced in PBMCs from LN patients and active SLE patients. Plasma IL-10 levels were significantly higher in LN patients than controls. IL-10R1 expression on CD4(+) T cells and signalling in PBMCs were down-regulated in LN patients, indicating that IL-10 and its receptor may have a special role in LN pathogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1365-2249
pubmed:author
pubmed:copyrightInfo
© 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology.
pubmed:issnType
Electronic
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-71
pubmed:meshHeading
pubmed-meshheading:21635228-Adolescent, pubmed-meshheading:21635228-Adult, pubmed-meshheading:21635228-CD4-Positive T-Lymphocytes, pubmed-meshheading:21635228-CD8-Positive T-Lymphocytes, pubmed-meshheading:21635228-Cells, Cultured, pubmed-meshheading:21635228-Cytokines, pubmed-meshheading:21635228-Down-Regulation, pubmed-meshheading:21635228-Female, pubmed-meshheading:21635228-Flow Cytometry, pubmed-meshheading:21635228-Humans, pubmed-meshheading:21635228-Interleukin-10, pubmed-meshheading:21635228-Leukocytes, Mononuclear, pubmed-meshheading:21635228-Lupus Erythematosus, Systemic, pubmed-meshheading:21635228-Lupus Nephritis, pubmed-meshheading:21635228-Male, pubmed-meshheading:21635228-Middle Aged, pubmed-meshheading:21635228-Phosphorylation, pubmed-meshheading:21635228-Receptors, Interleukin-10, pubmed-meshheading:21635228-STAT1 Transcription Factor, pubmed-meshheading:21635228-STAT3 Transcription Factor, pubmed-meshheading:21635228-Signal Transduction
pubmed:year
2011
pubmed:articleTitle
Interleukin-10 receptor expression and signalling were down-regulated in CD4? T cells of lupus nephritis patients.
pubmed:affiliation
Department of Rheumatology and Immunology, Shengjing Hospital, Shenyang, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't