Linked Life Data

21632112

Source:http://linkedlifedata.com/resource/pubmed/id/21632112

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15-16
pubmed:dateCreated
2011-8-29
pubmed:abstractText
To understand the interaction of dendritic cells (DCs) with cancer cells, we investigated molecular changes in DCs following co-culture with cancer cells. DCs co-cultured with Jurkat cancer cells showed remarkable down-regulation of MHC class I molecules, while DCs co-cultured with MCF-7 cancer cells showed minimal changes. Interestingly, down-regulation of MHC class I on DCs was not observed upon treatment with Jurkat cell lysate or culture supernatant, suggesting the importance of direct cell-cell interactions. The expressions of CD40, CD80, CD83, MHC class II, and IL-12p40 on DCs co-cultured with Jurkat cells were only slightly affected. In contrast, DCs co-cultured with MCF-7 cells showed increased expressions of CD80, CD83, CD86, and IL-12p40. Furthermore, DCs co-cultured with Jurkat cells showed a down-regulation of low molecular weight polypeptides (LMP) 7, and of transporter associated with antigen processing (TAP) 1 and 2 at the mRNA expression level. LMP7, TAP2 and ?2-microglobulin (?2M) were also down-regulated at the protein level. We further demonstrated how altered expression of MHC class I on DCs caused by co-culture with cancer cells affected autologous CD8(+) T cells, using the model MHC class I-presented HSV antigen. We found that DCs that had been HSV-treated and co-cultured with Jurkat cells showed a reduced potency to activate CD8(+) T cells. In contrast, HSV-treated DCs that had been co-cultured with MCF-7 cells induced activation of CD8(+) T cells, including high expression of CD25, CD69, granzyme B and cytokines, TNF-? and IFN-?.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1872-9142
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1744-52
pubmed:meshHeading
pubmed-meshheading:21632112-Antigen Presentation, pubmed-meshheading:21632112-Antigens, CD, pubmed-meshheading:21632112-Blotting, Western, pubmed-meshheading:21632112-CD8-Positive T-Lymphocytes, pubmed-meshheading:21632112-Cell Communication, pubmed-meshheading:21632112-Cell Line, Tumor, pubmed-meshheading:21632112-Coculture Techniques, pubmed-meshheading:21632112-Cytokines, pubmed-meshheading:21632112-Dendritic Cells, pubmed-meshheading:21632112-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:21632112-Histocompatibility Antigens Class I, pubmed-meshheading:21632112-Humans, pubmed-meshheading:21632112-Immunophenotyping, pubmed-meshheading:21632112-Lymphocyte Activation, pubmed-meshheading:21632112-Neoplasms, pubmed-meshheading:21632112-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2011
pubmed:articleTitle
Interactions of dendritic cells with cancer cells and modulation of surface molecules affect functional properties of CD8+ T cells.
pubmed:affiliation
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't