Linked Life Data

2163121

Source:http://linkedlifedata.com/resource/pubmed/id/2163121

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-8-1
pubmed:abstractText
The purpose of these studies was to determine whether extracellular calcium and calcium channels can influence chrysotile asbestos-stimulated production of superoxide anion by guinea pig alveolar macrophages. Increasing the extracellular calcium concentration from 0.0 to 10.0 mM markedly enhanced the ability of noncytotoxic levels of chrysotile (25 micrograms/ml) to stimulate macrophage production of superoxide anion at 37 degrees C. The primary effect of increasing extracellular calcium was to prolong superoxide anion production. In parallel with these calcium-dependent effects on superoxide anion production, chrysotile also caused an elevation of cytosolic calcium (measured using Fura-2) which increased with increasing extracellular calcium concentrations. A number of organic calcium channel antagonists were tested for their ability to block chrysotile-stimulated superoxide anion production by cells in medium containing physiological levels of calcium. The relative order of potency of these antagonists was verapamil much greater than nimopidine approximately diltiazem approximately nifedipine greater than segontin. In parallel with its effects on superoxide anion production, verapamil also partially blocked the observed chrysotile-stimulated elevation of cytosolic calcium. Taken together, these results suggest that chrysotile can open calcium channels on the macrophage surface, allowing extracellular calcium to enter and contribute to the elevation of cytosolic calcium levels. This elevation of cytosolic calcium in turn serves to prolong chrysotile-stimulated superoxide anion production.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
130-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Role of extracellular calcium in chrysotile asbestos stimulation of alveolar macrophages.
pubmed:affiliation
Department of Internal Medicine, University of Texas Medical School, Houston 77030.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't