Linked Life Data

21628893

Source:http://linkedlifedata.com/resource/pubmed/id/21628893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-6-1
pubmed:abstractText
The unripe fruits of Rubus coreanus (Rosaceae) are used in traditional Chinese medicine to relieve kidney dysfunction. In the present study, we evaluated the protective effects of the triterpenoid glycoside niga-ichigoside F? (NIF?) and of its aglycone 23-hydroxytormentic acid (23-HTA) isolated from the unripe fruits of Rubus coreanus (Rosaceae) against cisplatin-induced cytotoxicity in renal epithelial LLC-PK? cells. Pretreating LLC-PK? cells with 23-HTA or NIF? was found to prevent cisplatin-induced cytotoxicity and apoptosis. In addition, 23-HTA or NIF? pretreatment significantly improved the changes associated with cisplatin toxicity by increasing levels of glutathione (GSH) and decreasing levels of malondialdehyde (MDA) and reactive oxygen species (ROS). The activity of antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) was significantly lower in cisplatin-treated LL-PK? cells, and 23-HTA or NIF? treatment notably increased the these enzyme activity and protein and mRNA levels of CAT and manganese SOD (MnSOD). Moreover, cisplatin caused a significant decrease in nuclear levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and pretreatment with 23-HTA or NIF? significantly suppressed the cisplatin-induced translocation of Nrf2 in LLC-PK? cells. Taken together, these results suggest that 23-HTA ameliorates cisplatin-induced toxicity via modulation of antioxidant enzymes through activation of Nrf2 in LLC-PK? cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1347-5215
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
906-11
pubmed:meshHeading
pubmed-meshheading:21628893-Animals, pubmed-meshheading:21628893-Antineoplastic Agents, pubmed-meshheading:21628893-Antioxidants, pubmed-meshheading:21628893-Apoptosis, pubmed-meshheading:21628893-Cell Line, pubmed-meshheading:21628893-Cell Nucleus, pubmed-meshheading:21628893-Cell Survival, pubmed-meshheading:21628893-Cisplatin, pubmed-meshheading:21628893-Epithelial Cells, pubmed-meshheading:21628893-Fruit, pubmed-meshheading:21628893-Gene Expression Regulation, Enzymologic, pubmed-meshheading:21628893-Glutathione, pubmed-meshheading:21628893-Glycosides, pubmed-meshheading:21628893-Kidney, pubmed-meshheading:21628893-NF-E2-Related Factor 2, pubmed-meshheading:21628893-Oxidative Stress, pubmed-meshheading:21628893-Oxidoreductases, pubmed-meshheading:21628893-Protein Transport, pubmed-meshheading:21628893-RNA, Messenger, pubmed-meshheading:21628893-Rosaceae, pubmed-meshheading:21628893-Sus scrofa, pubmed-meshheading:21628893-Triterpenes
pubmed:year
2011
pubmed:articleTitle
23-Hydroxytormentic acid and niga-ichgoside f? isolated from Rubus coreanus attenuate cisplatin-induced cytotoxicity by reducing oxidative stress in renal epithelial LLC-PK? cells.
pubmed:affiliation
Department of Pharmaceutical Biochemistry and Department of Life and Nanopharmaceutical Science, Kyung Hee University, Seoul, Republic of Korea.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't