Linked Life Data

21628125

Source:http://linkedlifedata.com/resource/pubmed/id/21628125

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-6-1
pubmed:abstractText
The regenerative capacity of human interfollicular epidermis is closely linked to the potential of immature keratinocytes present within its basal layer. The availability of selection methods and culture systems allowing precise assessment of basal keratinocyte characteristics is critical for increasing our knowledge of this cellular compartment. This report presents a multi-parametric comparative study of basal keratinocytes selected according to two different principles: 1) high adhesion capacity on a type-I collagen-coated substrate [Adh???], 2) high cell-surface expression of ?6-integrin [Itg-?6 (high)]. Importantly, analysis performed at the single-cell level revealed similar primary clone-forming efficiency values of 45.5%?±?6.7% [Itg-?6(high)] and 43.7%?±?7.4% [Adh???], which were markedly higher than those previously reported. In addition, both methods selected keratinocytes exhibiting an extensive long-term growth potential exceeding 100 cell doublings and the capacity for generating a pluristratified epidermis. Our study also included a global transcriptome comparison. Genome-wide profiling indicated a strong similarity between [Adh???] and [Itg-?6(high)] keratinocytes, and revealed a common basal-associated transcriptional signature. In summary, cross-analysis of [Adh???] and [Itg-?6(high)] keratinocyte characteristics showed that these criteria identified highly equivalent cellular populations, both characterized by unexpectedly high growth capacities. These results may have broad impacts in the tissue engineering and cell therapy fields.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1167-1122
pubmed:author
pubmed:issnType
Print
pubmed:volume
21 Suppl 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12-20
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Cellular adhesion on collagen: a simple method to select human basal keratinocytes which preserves their high growth capacity.
pubmed:affiliation
Alternative Energies and Atomic Energy Commission, Institute of Cellular and Molecular Radiobiology, Laboratory of Genomics and Radiobiology of Keratinopoiesis, Evry Cedex, France. nicolas.fortunel@cea.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't