Linked Life Data

2162622

Source:http://linkedlifedata.com/resource/pubmed/id/2162622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-7-25
pubmed:abstractText
Ouabain-sensitive (OS) O2 consumption was determined in proximal tubular cells from weanling rats fed 21% (normal-protein, NP) or 50% (high-protein, HP) protein diet for 4 days. Butyric acid 10(-3)M was added as a substrate for mitochondrial respiration and the ionophore amphotericin B (10 micrograms ml-1) was used to sodium-load the cells. OS respiration was higher in HP than in NP cells in both DME and amino acid-free electrolyte solution (ES). Amphotericin B significantly increased OS respiration in both NP and HP cells, implying that the Na-K pump was activated by increased intracellular Na. In cells incubated in ES, addition of amino acids stimulated OS respiration significantly in HP cells (16.9 +/- 1.4 vs 21.2 +/- 1.1 nmol min-1 mg-1 protein) and in NP cells (13.9 +/- 0.3 vs 14.9 +/- 0.6 nmol min-1 mg-1 protein). Stimulation was significantly higher in HP cells (26 +/- 4%) than in NP cells (7 +/- 4%) (P less than 0.001). The amino acids did not stimulate ouabain-insensitive respiration. The results indicate that an HP diet to weanling rats will increase proximal tubule cell Na, K-ATPase-dependent respiration by enhancing Na entry via the Na-amino acid symports.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0001-6772
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-13
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Amino acid stimulation of Na,K-ATPase activity in rat proximal tubule after high-protein diet.
pubmed:affiliation
Department of Pediatrics, Huddinge Hospital, Sweden.
pubmed:publicationType
Journal Article