Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1990-7-26
pubmed:abstractText
Fluoroaluminate had no effect on cAMP levels in cells but inhibited agonist-stimulated cAMP accumulation. In membranes, fluoroaluminate stimulated adenylate cyclase activity between 1 and 10 mM but not at higher concentrations, and it inhibited agonist-stimulated adenylate cyclase activity at concentrations greater than 1 mM. Fluoroaluminate is known to activate Gs and Gi, the guanine nucleotide-binding (G) proteins that stimulate and inhibit adenylate cyclase. G proteins are heterotrimeric, with unique alpha and common beta gamma subunits, and activation involves dissociation of alpha from beta gamma. Pertussis toxin catalyzes ADP-ribosylation of alpha i of heterotrimeric Gi but not free alpha i. Fluoroaluminate prevented pertussis toxin-catalyzed ADP-ribosylation of Gi in cells and membranes, suggesting that Gi is activated by fluoroaluminate in both. Cholera toxin catalyzes ADP-ribosylation of the alpha s subunit of Gs. In cells, agonist often increased cholera toxin-catalyzed ADP-ribosylation of Gs, but fluoroaluminate decreased ADP-ribosylation even in the presence of agonist, suggesting that Gs cannot be activated in the presence of fluoroaluminate. In membranes, both agonist and fluoroaluminate increased cholera toxin-catalyzed ADP-ribosylation, suggesting that Gs is activated by these agents. We conclude that fluoroaluminate activates Gi but not Gs in cells and activates both G proteins in membranes. The value of bacterial toxins in assessing the state of G protein in cells and membranes is demonstrated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Aluminum, http://linkedlifedata.com/resource/pubmed/chemical/Aluminum Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Chlorides, http://linkedlifedata.com/resource/pubmed/chemical/Chorionic Gonadotropin, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP, http://linkedlifedata.com/resource/pubmed/chemical/Deferoxamine, http://linkedlifedata.com/resource/pubmed/chemical/Dibutyryl Cyclic GMP, http://linkedlifedata.com/resource/pubmed/chemical/Fluorine, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Sodium Fluoride, http://linkedlifedata.com/resource/pubmed/chemical/Theophylline, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella, http://linkedlifedata.com/resource/pubmed/chemical/aluminum chloride, http://linkedlifedata.com/resource/pubmed/chemical/fluoroaluminum
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10645-51
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:2162356-Adenylate Cyclase Toxin, pubmed-meshheading:2162356-Aluminum, pubmed-meshheading:2162356-Aluminum Compounds, pubmed-meshheading:2162356-Animals, pubmed-meshheading:2162356-Calcium, pubmed-meshheading:2162356-Cell Line, pubmed-meshheading:2162356-Cell Membrane, pubmed-meshheading:2162356-Cell Membrane Permeability, pubmed-meshheading:2162356-Chlorides, pubmed-meshheading:2162356-Chorionic Gonadotropin, pubmed-meshheading:2162356-Cyclic AMP, pubmed-meshheading:2162356-Deferoxamine, pubmed-meshheading:2162356-Dibutyryl Cyclic GMP, pubmed-meshheading:2162356-Fluorine, pubmed-meshheading:2162356-GTP-Binding Proteins, pubmed-meshheading:2162356-Humans, pubmed-meshheading:2162356-Kinetics, pubmed-meshheading:2162356-Pertussis Toxin, pubmed-meshheading:2162356-Phosphatidic Acids, pubmed-meshheading:2162356-Sodium Fluoride, pubmed-meshheading:2162356-Theophylline, pubmed-meshheading:2162356-Virulence Factors, Bordetella
pubmed:year
1990
pubmed:articleTitle
Differential activation of the stimulatory and inhibitory guanine nucleotide-binding proteins by fluoroaluminate in cells and in membranes.
pubmed:affiliation
Membrane Biochemistry Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article