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rdf:type |
|
|
lifeskim:mentions |
|
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pubmed:issue |
2
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pubmed:dateCreated |
1990-7-16
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pubmed:abstractText |
Cytochalasins induce little cytochrome c reduction in intact polymorphonuclear leukocytes (PMNs). A strong activation of the respiratory burst is induced by cytochalasin B or by the other cytochalasins, when the dye nitro blue tetrazolium (NBT) is used to measure the burst. Cytochalasin B-induced NBT reduction is dependent on calcium, which is mainly derived from intracellular stores. In the presence of NBT an enhanced binding of [3H]cytochalasin B can be observed. The enhanced reduction of NBT by cytochalasin B-treated cells is completely inhibited by pretreatment of the PMNs with pertussis toxin, suggesting the involvement of a pertussis toxin-sensitive G-protein in cytochalasin-induced activation of the respiratory burst.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
May
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|
pubmed:issn |
0034-5164
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
68
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
175-87
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2162072-Animals,
pubmed-meshheading:2162072-Cytochalasin B,
pubmed-meshheading:2162072-Cytochalasins,
pubmed-meshheading:2162072-Cytochrome c Group,
pubmed-meshheading:2162072-Neutrophils,
pubmed-meshheading:2162072-Nitroblue Tetrazolium,
pubmed-meshheading:2162072-Oxidation-Reduction,
pubmed-meshheading:2162072-Pertussis Toxin,
pubmed-meshheading:2162072-Rabbits,
pubmed-meshheading:2162072-Tetrazolium Salts,
pubmed-meshheading:2162072-Virulence Factors, Bordetella
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|
pubmed:year |
1990
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|
pubmed:articleTitle |
Cytochalasin-induced nitroblue tetrazolium dye (NBT) reduction in polymorphonuclear leukocytes.
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|
pubmed:affiliation |
Department of Medical Biochemistry, University of Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
In Vitro
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