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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6039
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pubmed:dateCreated |
2011-7-8
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pubmed:abstractText |
Selective autophagy can be mediated via receptor molecules that link specific cargoes to the autophagosomal membranes decorated by ubiquitin-like microtubule-associated protein light chain 3 (LC3) modifiers. Although several autophagy receptors have been identified, little is known about mechanisms controlling their functions in vivo. In this work, we found that phosphorylation of an autophagy receptor, optineurin, promoted selective autophagy of ubiquitin-coated cytosolic Salmonella enterica. The protein kinase TANK binding kinase 1 (TBK1) phosphorylated optineurin on serine-177, enhancing LC3 binding affinity and autophagic clearance of cytosolic Salmonella. Conversely, ubiquitin- or LC3-binding optineurin mutants and silencing of optineurin or TBK1 impaired Salmonella autophagy, resulting in increased intracellular bacterial proliferation. We propose that phosphorylation of autophagy receptors might be a general mechanism for regulation of cargo-selective autophagy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/OPTN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/SQSTM1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TBK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIIIA,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin,
http://linkedlifedata.com/resource/pubmed/chemical/light chain 3, human,
http://linkedlifedata.com/resource/pubmed/chemical/nuclear dot protein 52, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1095-9203
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pubmed:author |
pubmed-author:BradyNathan RNR,
pubmed-author:BumannDirkD,
pubmed-author:ChoudharyChunaramC,
pubmed-author:DötschVolkerV,
pubmed-author:DikicIvanI,
pubmed-author:FarhanHessoH,
pubmed-author:KoracJelenaJ,
pubmed-author:McEwanDavid GDG,
pubmed-author:RichterBenjaminB,
pubmed-author:RogovVladimir VVV,
pubmed-author:WagnerSebastianS,
pubmed-author:WaidmannOliverO,
pubmed-author:WildPhilippP
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pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
333
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
228-33
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pubmed:meshHeading |
pubmed-meshheading:21617041-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:21617041-Autophagy,
pubmed-meshheading:21617041-Cell Line, Tumor,
pubmed-meshheading:21617041-Cytosol,
pubmed-meshheading:21617041-HeLa Cells,
pubmed-meshheading:21617041-Humans,
pubmed-meshheading:21617041-Immunity, Innate,
pubmed-meshheading:21617041-Microtubule-Associated Proteins,
pubmed-meshheading:21617041-Models, Biological,
pubmed-meshheading:21617041-Nuclear Proteins,
pubmed-meshheading:21617041-Phosphorylation,
pubmed-meshheading:21617041-Protein Binding,
pubmed-meshheading:21617041-Protein Interaction Domains and Motifs,
pubmed-meshheading:21617041-Protein-Serine-Threonine Kinases,
pubmed-meshheading:21617041-RNA Interference,
pubmed-meshheading:21617041-Salmonella typhimurium,
pubmed-meshheading:21617041-Transcription Factor TFIIIA,
pubmed-meshheading:21617041-Ubiquitin
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pubmed:year |
2011
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pubmed:articleTitle |
Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth.
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pubmed:affiliation |
Frankfurt Institute for Molecular Life Sciences and Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt (Main), Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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