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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-7-11
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pubmed:databankReference |
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pubmed:abstractText |
The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA fragments and terminal sequences are amplified using a vector specific primer and a linker specific primer. Sequence data derived from these terminal specific products can be used to design primers for a further round of screening to isolate overlapping clones. The method also provides a convenient method of generating Sequence Tagged Sites for the mapping of complex genomes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-16593771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2186372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2542900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2544027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2668948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2678105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2771952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2781285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-2852134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-3033825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-3045566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-3047679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2161516-3262215
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2887-90
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:2161516-Base Sequence,
pubmed-meshheading:2161516-Chromosomes, Fungal,
pubmed-meshheading:2161516-Cloning, Molecular,
pubmed-meshheading:2161516-DNA Restriction Enzymes,
pubmed-meshheading:2161516-Gene Amplification,
pubmed-meshheading:2161516-Gene Library,
pubmed-meshheading:2161516-Genetic Techniques,
pubmed-meshheading:2161516-Genetic Vectors,
pubmed-meshheading:2161516-Molecular Sequence Data,
pubmed-meshheading:2161516-Restriction Mapping,
pubmed-meshheading:2161516-Saccharomyces cerevisiae
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pubmed:year |
1990
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pubmed:articleTitle |
A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.
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pubmed:affiliation |
ICI Pharmaceuticals, Biotechnology Department, Macclesfield, Cheshire, UK.
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pubmed:publicationType |
Journal Article
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