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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2011-6-17
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pubmed:abstractText |
Post-transcriptional regulation of mRNA includes restriction mechanisms to prevent export and expression of mRNAs that are incompletely spliced. Here we present evidence that the mammalian protein Tpr is involved in this restriction. To study the role of Tpr in export of mRNA with retained introns, we used reporters in which the mRNA was exported either via the Nxf1/Nxt1 pathway using a CTE or via the Crm1 pathway using Rev/RRE. Our data show that even modest knockdown of Tpr using RNAi leads to a significant increase in export and translation from the mRNA containing the CTE. In contrast, Tpr perturbation has no effect on export of mRNA containing the RRE, either in the absence or presence of Rev. Also, no effects were observed on export of a completely spliced mRNA. Taken together, our results indicate that Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/NXF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Pore Complex Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocytoplasmic Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TPR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/rev Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1469-9001
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1344-56
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pubmed:meshHeading |
pubmed-meshheading:21613532-Active Transport, Cell Nucleus,
pubmed-meshheading:21613532-Cell Nucleus,
pubmed-meshheading:21613532-Cells, Cultured,
pubmed-meshheading:21613532-Gene Expression,
pubmed-meshheading:21613532-Gene Knockdown Techniques,
pubmed-meshheading:21613532-HIV Core Protein p24,
pubmed-meshheading:21613532-Humans,
pubmed-meshheading:21613532-Introns,
pubmed-meshheading:21613532-Nuclear Pore Complex Proteins,
pubmed-meshheading:21613532-Nucleocytoplasmic Transport Proteins,
pubmed-meshheading:21613532-Proto-Oncogene Proteins,
pubmed-meshheading:21613532-RNA, Messenger,
pubmed-meshheading:21613532-RNA, Small Interfering,
pubmed-meshheading:21613532-RNA Precursors,
pubmed-meshheading:21613532-RNA-Binding Proteins,
pubmed-meshheading:21613532-Signal Transduction,
pubmed-meshheading:21613532-rev Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2011
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pubmed:articleTitle |
The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathway.
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pubmed:affiliation |
Myles H. Thaler Center for AIDS and Human Retrovirus Research and Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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