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rdf:type |
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lifeskim:mentions |
umls-concept:C0002638,
umls-concept:C0007634,
umls-concept:C0013303,
umls-concept:C0030274,
umls-concept:C0082420,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0205263,
umls-concept:C0242303,
umls-concept:C0851285,
umls-concept:C1332712,
umls-concept:C1366583,
umls-concept:C1521761,
umls-concept:C1655731
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pubmed:issue |
9
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pubmed:dateCreated |
2011-9-12
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pubmed:abstractText |
Pancreatic and duodenal homeobox factor 1 (PDX-1) maintains ?-cell function and differentiation via direct regulation of multiple islet cell genes. However, the molecular mechanisms involved in this process remain unknown. Here, we show that PDX-1 plays an important role in the induction of CD44+/CD105+ human amniotic fluid cells (HuAFCs) into functional pancreatic ?-cell-like cells in vitro. CD44+/CD105+ HuAFCs were transfected with either siRNA targeting PDX-1 (siRNA-PDX-1) or mock plasmid (siRNA-MOCK). Following induction, siRNA-MOCK-transfected cells differentiated into ?-cell-like cells that expressed multiple islet cell markers and produced insulin and C-peptide in a glucose-regulated manner. However, siRNA-PDX-1-transfected cells did not fully differentiate into ?-cell-like cells. Further, we observed epigenetic changes at the PDX-1 gene locus in induced CD44(+)/CD105(+) HuAFCs. Therefore, CD44+/CD105+ HuAFCs could be a source of human pancreatic ?-cell-like cells with potential uses in cell replacement therapy for diabetes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/C-Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/ENG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/pancreatic and duodenal homeobox 1...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1557-7430
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
739-48
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:21612404-Amniotic Fluid,
pubmed-meshheading:21612404-Antigens, CD,
pubmed-meshheading:21612404-Antigens, CD44,
pubmed-meshheading:21612404-Blotting, Western,
pubmed-meshheading:21612404-C-Peptide,
pubmed-meshheading:21612404-Cell Differentiation,
pubmed-meshheading:21612404-Chromatin Immunoprecipitation,
pubmed-meshheading:21612404-DNA Primers,
pubmed-meshheading:21612404-Epigenesis, Genetic,
pubmed-meshheading:21612404-Flow Cytometry,
pubmed-meshheading:21612404-Homeodomain Proteins,
pubmed-meshheading:21612404-Humans,
pubmed-meshheading:21612404-Insulin,
pubmed-meshheading:21612404-Insulin-Secreting Cells,
pubmed-meshheading:21612404-Microscopy, Fluorescence,
pubmed-meshheading:21612404-Oligonucleotides,
pubmed-meshheading:21612404-Polymerase Chain Reaction,
pubmed-meshheading:21612404-Promoter Regions, Genetic,
pubmed-meshheading:21612404-RNA, Small Interfering,
pubmed-meshheading:21612404-Receptors, Cell Surface,
pubmed-meshheading:21612404-Trans-Activators
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pubmed:year |
2011
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pubmed:articleTitle |
Induction of pancreatic ?-cell-like cells from CD44+/CD105+ human amniotic fluids via epigenetic regulation of the pancreatic and duodenal homeobox factor 1 promoter.
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pubmed:affiliation |
Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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