Linked Life Data

2161073

Source:http://linkedlifedata.com/resource/pubmed/id/2161073

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-6-29
pubmed:abstractText
Nine monoclonal antibodies (MAbs) directed to F protein of Sendai virus were obtained and characterized for their protective ability against Sendai virus infection in mice. None of the MAbs showed hemagglutination-inhibition (HI), hemolysis-inhibition (HLI), or neutralization (NT) activities in vitro when assayed by standard methods. Some of the MAbs, however, showed complement-requiring NT (C-NT) and complement-requiring hemolysis (C-HL) activities when assayed in the presence of complement. Passive immunization experiments revealed that the MAbs with higher C-NT and C-HL activities showed protective activity against Sendai virus pneumonia in mice, and that some MAbs with IgG1 isotype having neither C-NT nor C-HL activity also showed the protective activity. Digestion of the MAbs with pepsin which split immunoglobulin molecules into F(ab')2 and Fc fragments greatly suppressed the protective activity. These results suggest that not only complement-mediated immunological responses such as immune virolysis but also antibody-dependent cellular cytotoxicity (ADCC) and/or immune phagocytosis, in which complement system is not necessarily involved, play an important role in the protection of mice from Sendai virus infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0385-5600
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2161073-Animals, pubmed-meshheading:2161073-Antibodies, Monoclonal, pubmed-meshheading:2161073-Complement Hemolytic Activity Assay, pubmed-meshheading:2161073-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:2161073-Hemagglutination Tests, pubmed-meshheading:2161073-Immunization, Passive, pubmed-meshheading:2161073-Lethal Dose 50, pubmed-meshheading:2161073-Mice, pubmed-meshheading:2161073-Mice, Inbred BALB C, pubmed-meshheading:2161073-Muscle Proteins, pubmed-meshheading:2161073-Neutralization Tests, pubmed-meshheading:2161073-Oxidoreductases, pubmed-meshheading:2161073-Parainfluenza Virus 1, Human, pubmed-meshheading:2161073-Paramyxoviridae Infections, pubmed-meshheading:2161073-Pneumonia, pubmed-meshheading:2161073-Proteins, pubmed-meshheading:2161073-Radioimmunoprecipitation Assay
pubmed:year
1990
pubmed:articleTitle
Protection of mice against Sendai virus pneumonia by non-neutralizing anti-F monoclonal antibodies.
pubmed:affiliation
Department of Microbiology, Kobe University School of Medicine, Hyogo.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't