Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-7-3
pubmed:abstractText
We identified and characterized a new Escherichia coli gene, htrC. Inactivation of the htrC gene results in the inability to form colonies at 42 degrees C. An identical bacterial phenotype is found whether the htrC gene is inactivated either by Tn5 insertions or by a deletion spanning the entire gene. The htrC gene has been localized at 90 min, immediately downstream of the rpoC gene, and has been previously sequenced. It codes for a basic polypeptide with an Mr of 21,130. The htrC gene is under heat shock regulation, since it is transcribed actively only in bacteria possessing functional sigma 32. Inactivation of htrC results in (i) bacterial filamentation at intermediate temperatures, (ii) cell lysis at temperatures above 42 degrees C, (iii) overproduction of sigma 32-dependent heat shock proteins at all temperatures, (iv) overproduction of a few additional polypeptides, (v) underproduction of many polypeptides, and (vi) an overall defect in cellular proteolysis as judged by the reduced rate of puromycyl polypeptide degradation. In addition, the presence of an htrC mutation eliminates the UV sensitivity normally exhibited by lon mutant bacteria.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-173710, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2180903, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-23215, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2430947, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2497052, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2522091, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2531087, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2536191, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2537822, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2563997, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2651417, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2691330, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2900239, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-2999072, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3025174, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3038334, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3038679, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3149251, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3162770, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-333444, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3537305, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-368040, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3887408, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-3894330, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6098521, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6240590, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6285380, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6311435, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6315531, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6380765, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-6818556, http://linkedlifedata.com/resource/pubmed/commentcorrection/2160943-7038687
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3417-26
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A new Escherichia coli heat shock gene, htrC, whose product is essential for viability only at high temperatures.
pubmed:affiliation
Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.