Linked Life Data

21602602

Source:http://linkedlifedata.com/resource/pubmed/id/21602602

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-5-23
pubmed:abstractText
Herein, we investigated the possible mechanisms by which recombinant modified CRP(m(r)CRP) modulates blood platelet function. Modified CRP could activate blood platelets and stimulate their adhesion and aggregation in the absence of any other physiological stimuli. Preincubation of isolated blood platelets with m(r)CRP at a concentration as low as 2 ?g/ml resulted in significant platelet degranulation (fraction of CD62-positive platelets increased 2-fold, p < 0.0002), and at concentrations of 20 ?g/ml and 100 ?g/ml, increased exposure of the platelet procoagulant surface was observed (expression of annexin V-positive platelets increased to 5.7 ± 1.0% and 10.4 ± 2.2%, respectively, p < 0.03, vs. 2.9 ± 0.2% in control). Furthermore, m(r)CRP (100 ?g/ml) strongly augmented spontaneous and ADP-induced fibrinogen binding to platelets (p < 0.05), platelet adhesion to fibrinogen and platelet aggregation. Using the Biacore™ surface plasmon resonance technique and glycoprotein Ib? (GPIb?) immobilized on the sensor surface, we demonstrated direct binding between platelet GPIb? and m(r)CRP. Binding of m(r)CRP to GPIb? and C1q was also observed by ELISA, irrespective of the immobilized ligand. These outcomes strongly support a role of the GPIb-IX-V complex in the interactions of m(r)CRP with blood platelets.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1734-1140
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
464-75
pubmed:meshHeading
pubmed:articleTitle
Modified C-reactive protein interacts with platelet glycoprotein Ib?.
pubmed:affiliation
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, ?eromskiego 113, PL 90-549 ?ód?, Poland. mboncler@csk.umed.lodz.pl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't