Source:http://linkedlifedata.com/resource/pubmed/id/21601180
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2011-7-4
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| pubmed:abstractText |
Borrelia burgdorferi is the etiological agent for Lyme disease (LD), the most common vector borne disease in the United States. There is no human vaccine against LD currently available. Our approach to a vaccine is based on its surface-exposed glycolipids. One group of these glycolipids termed BBGL-2 consists of 1,2-di-O-acyl-3-O-(?-d-galactopyranosyl)-sn-glycerol congeners having palmitic, oleic, stearic, linoleic, and myristic acids. In order to delineate the immunodominant region(s) of the BBGL-2 components, we embarked on a synthetic project to provide available structurally defined, homogeneous analogs of BBGL-2 that might help identify the best vaccine candidate. The antigenicity of the synthetic glycolipids was examined by dot-blot analysis using mice sera obtained by immunization with killed B. burgdorferi cells, with native BBGL-2 in complete Freund's adjuvant, as well as sera obtained from patients with Lyme disease. We found that the presence of two acyl groups in the glycerol moiety was essential for antigenicity. At least one of these groups must be an oleoyl moiety. Neither the anomeric configuration of the galactose nor the configuration of the glycerol at C-2 was a decisive factor. Based on these findings we designed an 'unnatural' BBGL-2 analog having the structure 3-O-(?-d-galactopyranosyl)-1,2-di-O-oleoyl-dl-glycerol which is easier and less expensive to synthesize than the other BBGL-2 congeners prepared in this study. This substance proved to be antigenic and is considered a candidate vaccine for Lyme disease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Immunodominant Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1873-426X
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| pubmed:author | |
| pubmed:copyrightInfo |
Published by Elsevier Ltd.
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| pubmed:issnType |
Electronic
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| pubmed:day |
6
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| pubmed:volume |
346
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1551-63
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| pubmed:meshHeading |
pubmed-meshheading:21601180-Animals,
pubmed-meshheading:21601180-Antibodies, Bacterial,
pubmed-meshheading:21601180-Antigens, Bacterial,
pubmed-meshheading:21601180-Borrelia burgdorferi,
pubmed-meshheading:21601180-Chromatography, Thin Layer,
pubmed-meshheading:21601180-Diglycerides,
pubmed-meshheading:21601180-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:21601180-Glycolipids,
pubmed-meshheading:21601180-Humans,
pubmed-meshheading:21601180-Immunization,
pubmed-meshheading:21601180-Immunoblotting,
pubmed-meshheading:21601180-Immunodominant Epitopes,
pubmed-meshheading:21601180-Lyme Disease,
pubmed-meshheading:21601180-Magnetic Resonance Spectroscopy,
pubmed-meshheading:21601180-Mice,
pubmed-meshheading:21601180-Molecular Mimicry,
pubmed-meshheading:21601180-Oleic Acid,
pubmed-meshheading:21601180-Vaccines, Synthetic
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| pubmed:year |
2011
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| pubmed:articleTitle |
Synthesis and antigenicity of BBGL-2 glycolipids of Borrelia burgdorferi, the causative agent of Lyme disease.
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| pubmed:affiliation |
Program on Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-2423, United States. pozsgayv@mail.nih.gov
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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