Linked Life Data

21600900

Source:http://linkedlifedata.com/resource/pubmed/id/21600900

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:21600900rdf:typepubmed:Citationlld:pubmed
pubmed-article:21600900lifeskim:mentionsumls-concept:C0031715lld:lifeskim
pubmed-article:21600900lifeskim:mentionsumls-concept:C0013936lld:lifeskim
pubmed-article:21600900lifeskim:mentionsumls-concept:C0037119lld:lifeskim
pubmed-article:21600900lifeskim:mentionsumls-concept:C1314939lld:lifeskim
pubmed-article:21600900lifeskim:mentionsumls-concept:C0323309lld:lifeskim
pubmed-article:21600900pubmed:issue7lld:pubmed
pubmed-article:21600900pubmed:dateCreated2011-7-5lld:pubmed
pubmed-article:21600900pubmed:abstractTextPhosphorylation of the translational repressor 4E-binding protein (4E-BP) plays a critical role in regulating the overall translation levels in cells. In the present study, we investigated 4E-BP phosphorylation of Bombyx mori eggs by an immunoblot analysis of a conserved phospho-specific antibody to 4E-BP and demonstrated its role during embryonic development. When HCl treatment was applied to diapause-destined eggs at 20 h after oviposition, a dramatic increase in the phosphorylation of 4E-BP occurred 5 min after treatment with HCl, and high phosphorylation levels were maintained throughout embryonic stage in HCl-treated eggs compared to those in diapause (control) eggs. When HCl treatment was applied to diapause eggs on day 10 after oviposition, no dramatic activation in 4E-BP phosphorylation occurred, indicating stage-specific effects of HCl treatment. In both non-diapause eggs and eggs whose diapause had been terminated by chilling of diapausing eggs at 5°C for 70 days and then were transferred to 25°C, high phosphorylation levels of 4E-BP were also detected. Moreover, 4E-BP phosphorylation dramatically increased when dechorionated eggs were incubated in medium. The addition of rapamycin, a specific inhibitor of mammalian target of rapamycin (TOR) signaling, and LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, but not the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor, U0126, dose-dependently inhibited 4E-BP phosphorylation in dechorionated eggs, indicating that PI3K/TOR signaling is an upstream signaling event involved in 4E-BP phosphorylation. Examination of 4E-BP gene expression levels showed no differences between treatments with HCl and water in the first hour after treatment, indicating that changes in phosphorylation of 4E-BP upon HCl treatment are mainly regulated at the post-transcriptional level. In addition, MAPK pathways and glycogen synthase kinase (GSK)-3? phosphorylation were not significantly affected in the first hour after HCl treatment. These results demonstrate that the rapid phosphorylation of 4E-BP is an early signaling event in embryonic development in the eggs whose diapause initiation was prevented by HCl treatment, thus being involved in the embryonic development of B. mori.lld:pubmed
pubmed-article:21600900pubmed:languageenglld:pubmed
pubmed-article:21600900pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:citationSubsetIMlld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21600900pubmed:statusMEDLINElld:pubmed
pubmed-article:21600900pubmed:monthJullld:pubmed
pubmed-article:21600900pubmed:issn1879-1611lld:pubmed
pubmed-article:21600900pubmed:authorpubmed-author:GuShi-HongSHlld:pubmed
pubmed-article:21600900pubmed:authorpubmed-author:YoungShun-Chi...lld:pubmed
pubmed-article:21600900pubmed:authorpubmed-author:LinJu-LingJLlld:pubmed
pubmed-article:21600900pubmed:authorpubmed-author:TsaiWen-Hsien...lld:pubmed
pubmed-article:21600900pubmed:authorpubmed-author:LinPei-LingPLlld:pubmed
pubmed-article:21600900pubmed:copyrightInfoCopyright © 2011 Elsevier Ltd. All rights reserved.lld:pubmed
pubmed-article:21600900pubmed:issnTypeElectroniclld:pubmed
pubmed-article:21600900pubmed:volume57lld:pubmed
pubmed-article:21600900pubmed:ownerNLMlld:pubmed
pubmed-article:21600900pubmed:authorsCompleteYlld:pubmed
pubmed-article:21600900pubmed:pagination978-85lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:meshHeadingpubmed-meshheading:21600900...lld:pubmed
pubmed-article:21600900pubmed:year2011lld:pubmed
pubmed-article:21600900pubmed:articleTitleInvolvement of 4E-BP phosphorylation in embryonic development of the silkworm, Bombyx mori.lld:pubmed
pubmed-article:21600900pubmed:affiliationDepartment of Zoology, National Museum of Natural Science, 1 Kuan-Chien Road, Taichung 404, Taiwan, ROC. gu330@mail.nmns.edu.twlld:pubmed
pubmed-article:21600900pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21600900pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
entrez-gene:732945entrezgene:pubmedpubmed-article:21600900lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:21600900lld:entrezgene