Source:http://linkedlifedata.com/resource/pubmed/id/21600900
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2011-7-5
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| pubmed:abstractText |
Phosphorylation of the translational repressor 4E-binding protein (4E-BP) plays a critical role in regulating the overall translation levels in cells. In the present study, we investigated 4E-BP phosphorylation of Bombyx mori eggs by an immunoblot analysis of a conserved phospho-specific antibody to 4E-BP and demonstrated its role during embryonic development. When HCl treatment was applied to diapause-destined eggs at 20 h after oviposition, a dramatic increase in the phosphorylation of 4E-BP occurred 5 min after treatment with HCl, and high phosphorylation levels were maintained throughout embryonic stage in HCl-treated eggs compared to those in diapause (control) eggs. When HCl treatment was applied to diapause eggs on day 10 after oviposition, no dramatic activation in 4E-BP phosphorylation occurred, indicating stage-specific effects of HCl treatment. In both non-diapause eggs and eggs whose diapause had been terminated by chilling of diapausing eggs at 5°C for 70 days and then were transferred to 25°C, high phosphorylation levels of 4E-BP were also detected. Moreover, 4E-BP phosphorylation dramatically increased when dechorionated eggs were incubated in medium. The addition of rapamycin, a specific inhibitor of mammalian target of rapamycin (TOR) signaling, and LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, but not the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor, U0126, dose-dependently inhibited 4E-BP phosphorylation in dechorionated eggs, indicating that PI3K/TOR signaling is an upstream signaling event involved in 4E-BP phosphorylation. Examination of 4E-BP gene expression levels showed no differences between treatments with HCl and water in the first hour after treatment, indicating that changes in phosphorylation of 4E-BP upon HCl treatment are mainly regulated at the post-transcriptional level. In addition, MAPK pathways and glycogen synthase kinase (GSK)-3? phosphorylation were not significantly affected in the first hour after HCl treatment. These results demonstrate that the rapid phosphorylation of 4E-BP is an early signaling event in embryonic development in the eggs whose diapause initiation was prevented by HCl treatment, thus being involved in the embryonic development of B. mori.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz...,
http://linkedlifedata.com/resource/pubmed/chemical/Butadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Chromones,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrochloric Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Morpholines,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Sirolimus,
http://linkedlifedata.com/resource/pubmed/chemical/TOR Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/U 0126,
http://linkedlifedata.com/resource/pubmed/chemical/cup protein, Drosophila
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1879-1611
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
57
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
978-85
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| pubmed:meshHeading |
pubmed-meshheading:21600900-Amino Acid Sequence,
pubmed-meshheading:21600900-Animals,
pubmed-meshheading:21600900-Bombyx,
pubmed-meshheading:21600900-Butadienes,
pubmed-meshheading:21600900-Chromones,
pubmed-meshheading:21600900-Drosophila Proteins,
pubmed-meshheading:21600900-Embryonic Development,
pubmed-meshheading:21600900-Enzyme Inhibitors,
pubmed-meshheading:21600900-Gene Expression Regulation, Developmental,
pubmed-meshheading:21600900-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:21600900-Hydrochloric Acid,
pubmed-meshheading:21600900-Immunoblotting,
pubmed-meshheading:21600900-Metamorphosis, Biological,
pubmed-meshheading:21600900-Mitogen-Activated Protein Kinases,
pubmed-meshheading:21600900-Morpholines,
pubmed-meshheading:21600900-Nitriles,
pubmed-meshheading:21600900-Ovum,
pubmed-meshheading:21600900-Phosphatidylinositol 3-Kinase,
pubmed-meshheading:21600900-Phosphorylation,
pubmed-meshheading:21600900-Signal Transduction,
pubmed-meshheading:21600900-Sirolimus,
pubmed-meshheading:21600900-TOR Serine-Threonine Kinases,
pubmed-meshheading:21600900-Time Factors
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| pubmed:year |
2011
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| pubmed:articleTitle |
Involvement of 4E-BP phosphorylation in embryonic development of the silkworm, Bombyx mori.
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| pubmed:affiliation |
Department of Zoology, National Museum of Natural Science, 1 Kuan-Chien Road, Taichung 404, Taiwan, ROC. gu330@mail.nmns.edu.tw
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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