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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-6-21
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pubmed:abstractText |
We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at approximately 1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3' kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2432394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2441878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2441879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2447085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2452398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2457254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2466336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2467744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2472218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2472219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2542286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2549489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2550144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2550825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2551507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2553693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2556641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2732223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2808338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2823265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2825654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2833705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2838326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2987699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-2991221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-3001732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-3030297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-3039909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-313931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-3494731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-3500513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-3500949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-4354860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-4355377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-526298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6090450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6090945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6093098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6095092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6157683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6166387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6272837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6312092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6326105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6334806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6410286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-6605482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-7358670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2160078-977646
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3816-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2160078-1-Phosphatidylinositol 4-Kinase,
pubmed-meshheading:2160078-Animals,
pubmed-meshheading:2160078-Antibodies,
pubmed-meshheading:2160078-Cell Line,
pubmed-meshheading:2160078-Enzyme Activation,
pubmed-meshheading:2160078-Epidermal Growth Factor,
pubmed-meshheading:2160078-Humans,
pubmed-meshheading:2160078-Immunoblotting,
pubmed-meshheading:2160078-Isoenzymes,
pubmed-meshheading:2160078-Kinetics,
pubmed-meshheading:2160078-Phosphotransferases,
pubmed-meshheading:2160078-Receptor, Epidermal Growth Factor,
pubmed-meshheading:2160078-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation.
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pubmed:affiliation |
Department of Medical Biochemistry, University of Calgary, Canada.
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pubmed:publicationType |
Journal Article
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