Linked Life Data

21598137

Source:http://linkedlifedata.com/resource/pubmed/id/21598137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2011-7-27
pubmed:abstractText
An indirect competitive enzyme-linked immunosorbent assay (ELISA) method using a monoclonal antibody for deoxynivalenol (DON) detection in wheat and flour was standardised and validated (detection limit?=?177.1?µg?kg(-1)) and its performance was compared with LC-MS, quantification limit?=140?µg?kg(-1)). DON recovery ranged from 88.7% to 122.6% for wheat grain and from 70.6% to 139.3% for flour. Among the 38 wheat samples evaluated, DON was detected in 29 samples (76.3%) by ic-ELISA (281.6-12?291.4?µg?kg(-1)) and in 22 samples (57.9%) by LC-MS (155.3-9906.9?µg?kg(-1)). The 0.93 correlation coefficient between ic-ELISA and LC-MS data in 19 positive DON wheat samples demonstrated the reliability and efficiency of ic-ELISA. Results indicated that standardised ic-ELISA was suitable for DON screening in wheat samples and the need for continuous monitoring of mycotoxin levels in foodstuffs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1944-0057
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1083-90
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Immunoassay based on monoclonal antibodies versus LC-MS: deoxynivalenol in wheat and flour in Southern Brazil.
pubmed:affiliation
Department of Food Science and Technology, Center of Agrarian Sciences, State University of Londrina, 86051-980, Londrina, Paraná, Brazil. joice.sifuentes@gmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't