Linked Life Data

2159803

Source:http://linkedlifedata.com/resource/pubmed/id/2159803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-6-21
pubmed:abstractText
A monoclonal antibody (mAb 95-111) was used to titrate the amounts of H+/K(+)-ATPase in subcellular fractions of the fundus of rats, pigs, rabbits and humans. All four had similar amounts of H+/K(+)-ATPase: 2.1 +/- 0.5 (human), 1.9 +/- 0.4 (rabbit), 4.4 +/- 0.5 (rat) and 4.2 +/- 0.8 (hog) mg ATPase/g wet tissue. The antigen concentrations and H+/K(+)-ATPase enzymatic activities of subcellular fractions were linearly correlated in all species but rat suggesting that human, rabbit and hog H+/K(+)-ATPases have similar rates of catalysis (223 mumol Pi/h per mg ATPase). The non-correlation of rat data probably reflects the known lability of the rat enzyme. Hence, immuno-titration promises to be a more reliable method of estimating rat ATPase than the currently used enzymatic assay. The H+/K(+)-ATPase content of human biopsies was 20-fold higher than previously-published (Smolka, A. and Weinstein, W.M. (1986) Gastroenterology 90, 532-539) suggesting that the previous immuno-titration underestimated the H+/K(+)-ATPase content of the human fundus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
1024
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
H+/K(+)-ATPase contents of human, rabbit, hog and rat gastric mucosa.
pubmed:affiliation
INSERM Unité 10, Ancien Hôpital Bichat, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study