Linked Life Data

21593350

Source:http://linkedlifedata.com/resource/pubmed/id/21593350

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-6-21
pubmed:abstractText
The incidence of inflammatory bowel diseases has increased during recent decades. Within the colon, the families of mucins (MUC) and trefoil factors (TFF) facilitate mucosal protection. Probiotic administration influences the intestinal MUC layer. Additionally, food components may affect gut microflora or have direct effects on the MUC barrier. Our objective was to determine whether diet and/or Lactobacillus rhamnosus GG (LGG) would mediate dextran sodium sulfate (DSS)-induced colitis by altering expression of the MUC and TFF genes. C57BL/6 mice were fed diets containing 20% (wt:wt) casein, soy, or whey proteins with or without LGG for 12 d. Seven days after starting LGG diets, the mice were given 2% DSS in drinking water for 4 d. Two additional casein groups with or without LGG were given tap water, for a total of 8 groups. One day after the DSS treatment, the mice were killed and the colon and cecum tissues and cecum contents were collected and analyzed by qRT-PCR. Whey protein significantly increased cecal LGG content compared with the other diets. In the casein diet groups, MUC1 and TFF-3 expression in colon was significantly induced by DSS independent of LGG. Compared with other DSS-treated groups, soy protein decreased MUC-1 and TFF-3 in the colon. Similarly, soy protein decreased the impact of DSS on inflammatory scores, TNF? gene expression, and colon shortening. There was no overall effect of LGG on these measurements. In conclusion, soy protein suppressed the DSS-induced inflammatory stimulation of MUC, TFF, and TNF? gene expression independently of LGG.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1541-6100
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1239-46
pubmed:meshHeading
pubmed-meshheading:21593350-Animals, pubmed-meshheading:21593350-Base Sequence, pubmed-meshheading:21593350-Caseins, pubmed-meshheading:21593350-Cecum, pubmed-meshheading:21593350-Colitis, Ulcerative, pubmed-meshheading:21593350-Colon, pubmed-meshheading:21593350-Dextran Sulfate, pubmed-meshheading:21593350-Disease Models, Animal, pubmed-meshheading:21593350-Female, pubmed-meshheading:21593350-Gene Expression, pubmed-meshheading:21593350-Lactobacillus rhamnosus, pubmed-meshheading:21593350-Mice, pubmed-meshheading:21593350-Mice, Inbred C57BL, pubmed-meshheading:21593350-Milk Proteins, pubmed-meshheading:21593350-Mucin-1, pubmed-meshheading:21593350-Mucins, pubmed-meshheading:21593350-Probiotics, pubmed-meshheading:21593350-RNA, Messenger, pubmed-meshheading:21593350-Soybean Proteins, pubmed-meshheading:21593350-Tumor Necrosis Factor-alpha
pubmed:year
2011
pubmed:articleTitle
Soy protein diet, but not Lactobacillus rhamnosus GG, decreases mucin-1, trefoil factor-3, and tumor necrosis factor-? in colon of dextran sodium sulfate-treated C57BL/6 mice.
pubmed:affiliation
Interdepartmental Graduate Program in Nutritional Sciences, Iowa State University, Ames, IA 50011, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't