Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-5-30
pubmed:abstractText
1. The roles of intracellular and extracellular-derived Ca2+ in alpha-adrenoceptor-mediated contractions to noradrenaline (NA) have been investigated in several isolated blood vessels from the rabbit by examining responses in the presence of a modified Krebs-Henseleit saline with 2.5 mM Ca2+ and a Ca2(+)-buffered saline with 0.1 microM free Ca2+. 2. NA was tested in preparations of the abdominal aorta, distal saphenous artery, renal vein, lateral saphenous vein, plantaris vein and ear vein exposed to a Ca2(+)-buffered saline with 0.1 microM [Ca2+]. A concentration of NA which was maximally effective in modified Krebs-Henseleit saline, produced an initial transient contraction (ITC) followed by a relaxation towards baseline. This is evidence that alpha-adrenoceptor-mediated responses in all these blood vessels depend upon calcium from both sources. 3. The ITC was particularly pronounced in the arteries and was associated more closely with the alpha 1-receptor subtype. In the abdominal aorta, distal saphenous artery and renal vein the ITC can almost exclusively be attributed to an alpha 1-adrenoceptor (prazosin-sensitive, rauwolscine-resistant). In the ear vein, and to a lesser extent the plantaris vein, the ITC was mediated in part by an alpha 2-adrenoceptor (prazosin-resistant, rauwolscine-sensitive). 4. alpha 2-Adrenoceptors in the lateral saphenous vein largely account for the response to NA in modified Krebs-Henseleit saline, but alpha 1-adrenoceptors mediate the ITC in Ca2(+)-buffered saline. After selective inactivation of alpha 1-adrenoceptors with a combination of phenoxybenzamine and rauwolscine, responses to NA in modified Krebs-Henseleit saline are slow in onset and there is no ITC in Ca2(+)-buffered saline. 5. The possible significance of the coupling of postjunctional alpha 2-adrenoceptors to dual sources of Ca2 + is discussed in relation to the interaction between alpha-adrenoceptor subtypes and the ease of demonstrating functional alpha 2-adrenoceptors in isolated blood vessels.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-1189935, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-228207, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2408777, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2441781, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2451639, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2564291, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2564396, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2840157, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2845052, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2852522, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2854670, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2857610, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2863372, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2870409, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2873236, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2880628, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2883187, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2886168, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2887026, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2906557, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2906558, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2989502, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-2992478, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-3022313, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-3668857, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-37533, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-4367920, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6096728, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6110451, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6138712, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6145472, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6248704, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6279105, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6284627, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6296369, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6322894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2158371-6421170
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-60
pubmed:dateRevised
2010-3-22
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
An examination of the sources of calcium for contractions mediated by postjunctional alpha 1- and alpha 2-adrenoceptors in several blood vessels isolated from the rabbit.
pubmed:affiliation
Institute of Physiology, University of Glasgow, Scotland.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't