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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1990-5-16
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pubmed:abstractText |
The SUV3-1 mutation was isolated earlier as a suppressor of a deletion of a conserved RNA processing site (dodecamer) near the 3' end of the var1 gene. Previous studies indicate that the suppressor enhances translation of mutant var1 messages; unexpectedly, it also causes over-accumulation of excised intron RNA of the large rRNA gene intron and blocks cleavage at the dodecamer site within that intron. In this study most mitochondrial genes in SUV3-1 and suv3 nuclear contexts are surveyed for changes in levels of mRNA, for interference with dodecamer cleavage and splicing and for levels of excised intron RNAs. SUV3-1 has little or no effect on the size or abundance of unspliced RNAs tested. It results, however, in a marked increase in the abundance of seven of eight excised group I intron RNAs tested, most of which are not detectable in wild-type (suv3) strains. The suppressor lowers levels of the cob and coxl mRNAs about 2-5 and 20-fold, respectively. The effect on coxl mRNA results from a decrease in the splicing of its intron 5 beta. Despite the reduction in these mRNA levels, the amounts of coxl and cyt b polypeptides were close to wild-type levels in SUV3-1 cells. These data show that the suv3 gene plays a prominent role in post-transcriptional and translation events in yeast mitochondria.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2427014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2479980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2536593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2645524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2657398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2833749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-2834089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3004738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3034607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3099165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3302679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3309947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-367368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-379518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3886160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3886163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-3886164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6188839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6210151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6253454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6254986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6273949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6281278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6297792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6313214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6315717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6315719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6325458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6327291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6330366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6348045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6370993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6760126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6986989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-6986990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-7042709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2158076-7043464
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1369-76
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2158076-Cell Nucleus,
pubmed-meshheading:2158076-Cytochrome b Group,
pubmed-meshheading:2158076-DNA, Ribosomal,
pubmed-meshheading:2158076-Electron Transport Complex IV,
pubmed-meshheading:2158076-Genes, Fungal,
pubmed-meshheading:2158076-Introns,
pubmed-meshheading:2158076-Mitochondria,
pubmed-meshheading:2158076-Mutation,
pubmed-meshheading:2158076-RNA, Messenger,
pubmed-meshheading:2158076-RNA, Ribosomal,
pubmed-meshheading:2158076-RNA Processing, Post-Transcriptional,
pubmed-meshheading:2158076-Restriction Mapping,
pubmed-meshheading:2158076-Saccharomyces cerevisiae
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pubmed:year |
1990
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pubmed:articleTitle |
The nuclear SUV3-1 mutation affects a variety of post-transcriptional processes in yeast mitochondria.
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pubmed:affiliation |
Department of Molecular Genetics, Ohio State University, Columbus 43210-1292.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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