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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1990-5-24
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pubmed:abstractText |
In polyomavirus-transformed cells, pp60c-src is activated by association with polyomavirus middle T antigen. These complexes have a higher tyrosine kinase activity compared with that of unassociated pp60c-src. Genetic analyses have revealed that the carboxy-terminal 15 amino acids of pp60c-src and the amino-terminal half of middle T antigen are required for this association and consequent activation of the tyrosine kinase. To define in greater detail the borders of the domain in middle T antigen required for activation of pp60c-src, we constructed a set of unidirectional amino-terminal deletion mutants of middle T antigen. Analysis of these mutants revealed that the first six amino acids of middle T antigen are required for it to activate the kinase activity of pp60c-src and to transform Rat-1 fibroblasts. Analysis of a series of insertion and substitution mutants confirmed these observations and further revealed that mutations affecting the first four amino acids of middle T antigen reduced or abolished its capacity to activate the kinase activity of pp60c-src and to transform Rat-1 cells in culture. Our results suggest that the first four amino acids of middle T antigen constitute part of a domain required for activation of the pp60c-src tyrosyl kinase activity and for consequent cellular transformation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-229972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-229973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-229974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2411538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2423706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2431281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2433463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2441878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2441879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2445489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2551672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2850472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2981329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-2987799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3007666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3011402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3023895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3029418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3103925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3103926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3103927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3345558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-4318787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6091061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6091900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6092671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6169848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6179082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6184609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6243123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6265803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6283174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6284958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6292832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6293767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6294500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6298462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6304524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6308461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6321957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6325177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6325896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6328442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6694911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157858-6700727
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1879-87
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2157858-Amino Acid Sequence,
pubmed-meshheading:2157858-Animals,
pubmed-meshheading:2157858-Antigens, Polyomavirus Transforming,
pubmed-meshheading:2157858-Base Sequence,
pubmed-meshheading:2157858-Cell Line,
pubmed-meshheading:2157858-Cell Transformation, Neoplastic,
pubmed-meshheading:2157858-Chromosome Deletion,
pubmed-meshheading:2157858-Molecular Sequence Data,
pubmed-meshheading:2157858-Mutation,
pubmed-meshheading:2157858-Oligonucleotide Probes,
pubmed-meshheading:2157858-Plasmids,
pubmed-meshheading:2157858-Polyomavirus,
pubmed-meshheading:2157858-Protein-Tyrosine Kinases,
pubmed-meshheading:2157858-Rats,
pubmed-meshheading:2157858-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
The amino terminus of polyomavirus middle T antigen is required for transformation.
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pubmed:affiliation |
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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