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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-5-24
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pubmed:abstractText |
In isolated rat pancreatic islets, the effect of diamide and N-ethylmaleimide (NEM) on forskolin- as well as on glucagon-induced elevation of cAMP was studied. Forskolin and glucagon increased cAMP levels in batch-incubated islets. Diamide and NEM further augmented forskolin-induced increase of cAMP levels, whereas glucagon-stimulated elevation of cAMP was not affected. From our data it is likely that under the conditions of the present study, the thiols related to the Ns-protein and the catalytic unit are insensitive to oxidation and alkylation. The potentiation of forskolin-induced cAMP production in intact islet cells by diamide and NEM may be due to inactivation of the thiols related to the Ni-protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0018-5043
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
61-4
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pubmed:dateRevised |
2009-2-19
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pubmed:meshHeading |
pubmed-meshheading:2157653-Animals,
pubmed-meshheading:2157653-Azo Compounds,
pubmed-meshheading:2157653-Cyclic AMP,
pubmed-meshheading:2157653-Diamide,
pubmed-meshheading:2157653-Drug Synergism,
pubmed-meshheading:2157653-Enzyme Induction,
pubmed-meshheading:2157653-Ethylmaleimide,
pubmed-meshheading:2157653-Forskolin,
pubmed-meshheading:2157653-Glucagon,
pubmed-meshheading:2157653-Islets of Langerhans,
pubmed-meshheading:2157653-Rats
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pubmed:year |
1990
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pubmed:articleTitle |
Potentiation of forskolin-induced increase of cAMP by diamide and N-ethylmaleimide in rat pancreatic islets.
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pubmed:affiliation |
Pharmazeutisches Institut, Abteilung Pharmakologie, Universität Tübingen, Germany.
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pubmed:publicationType |
Journal Article
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