Linked Life Data

21572391

Source:http://linkedlifedata.com/resource/pubmed/id/21572391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2011-7-6
pubmed:databankReference
pubmed:abstractText
Oestrogen receptor ? (ER?) is key player in the progression of breast cancer. Recently, the cistrome and interactome of ER? were mapped in breast cancer cells, revealing the importance of spatial organization in oestrogen-mediated transcription. However, the underlying mechanism of this process is unclear. Here, we show that ER? binding sites (ERBS) identified from the Chromatin Interaction Analysis-Paired End DiTag of ER? are enriched for AP-2 motifs. We demonstrate the transcription factor, AP-2?, which has been implicated in breast cancer oncogenesis, binds to ERBS in a ligand-independent manner. Furthermore, perturbation of AP-2? expression impaired ER? DNA binding, long-range chromatin interactions, and gene transcription. In genome-wide analyses, we show that a large number of AP-2? and ER? binding events converge together across the genome. The majority of these shared regions are also occupied by the pioneer factor, FoxA1. Molecular studies indicate there is functional interplay between AP-2? and FoxA1. Finally, we show that most ERBS associated with long-range chromatin interactions are colocalized with AP-2? and FoxA1. Together, our results suggest AP-2? is a novel collaborative factor in ER?-mediated transcription.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1460-2075
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2569-81
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
AP-2? regulates oestrogen receptor-mediated long-range chromatin interaction and gene transcription.
pubmed:affiliation
Cancer Biology and Pharmacology, Genome Institute of Singapore, A STAR (Agency for Science, Technology and Research), Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't