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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1990-5-2
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pubmed:abstractText |
Viral induction of the human beta-interferon (IFN-beta) gene leads to a transient accumulation of high levels of IFN-beta mRNA. Previous studies have shown that the increase in IFN-beta mRNA levels after induction is due to an increase in the rate of IFN-beta gene transcription. In this paper, we show that the rapid postinduction decrease in the level of IFN-beta mRNA is due to a combination of transcriptional repression and rapid turnover of the mRNA. This transcriptional repression can be blocked with cycloheximide, suggesting that the synthesis of a virus-inducible repressor is necessary for the postinduction turnoff of the IFN-beta gene. Analysis of the sequence requirements for IFN-beta mRNA instability revealed two regions capable of destabilizing a heterologous mRNA. One destabilizer is an AU-rich sequence in the 3' untranslated region, and the other is located 5' to the translation stop codon.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2414012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2419912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2455262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2467747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2496006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2540528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2672333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-269400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-270686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-2814487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3026904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3034597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3060261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3085953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3132954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3194021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3211141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3281258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3296191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3325826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3371658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3380094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3498164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3551944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3708688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3708689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3773893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3785197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-3986910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-419694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6090941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6091048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6174973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6313211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6330726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6575385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-6700582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-685176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2157136-856944
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1329-37
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2157136-Animals,
pubmed-meshheading:2157136-Base Sequence,
pubmed-meshheading:2157136-Cell Line,
pubmed-meshheading:2157136-Cell Transformation, Viral,
pubmed-meshheading:2157136-Cells, Cultured,
pubmed-meshheading:2157136-Cycloheximide,
pubmed-meshheading:2157136-Gene Expression Regulation,
pubmed-meshheading:2157136-Interferon Type I,
pubmed-meshheading:2157136-Molecular Sequence Data,
pubmed-meshheading:2157136-Parainfluenza Virus 1, Human,
pubmed-meshheading:2157136-Plasmids,
pubmed-meshheading:2157136-Poly I-C,
pubmed-meshheading:2157136-RNA, Messenger,
pubmed-meshheading:2157136-Transcription, Genetic,
pubmed-meshheading:2157136-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Postinduction turnoff of beta-interferon gene expression.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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