Source:http://linkedlifedata.com/resource/pubmed/id/21566130
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
27
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| pubmed:dateCreated |
2011-7-4
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| pubmed:abstractText |
Proepithelin, a previously unrecognized growth factor in cartilage, has recently emerged as an important regulator for cartilage formation and function. In the present study, we provide several lines of evidences in proepithelin-mediated induction of cell proliferation, differentiation, and apoptosis in the metatarsal growth plate. Proepithelin-mediated stimulation of metatarsal growth and growth plate chondrogenesis was neutralized by pyrrolidine dithiocarbamate, a known NF-?B inhibitor. In rat growth plate chondrocytes, proepithelin induced NF-?B-p65 nuclear translocation, and nuclear NF-?B-p65 initiated its target gene cyclin D1 to regulate chondrocyte functions. The inhibition of NF-?B-p65 expression and activity (by p65 short interfering RNA (siRNA) and pyrrolidine dithiocarbamate, respectively) in chondrocytes reversed the proepithelin-mediated induction of cell proliferation and differentiation and the proepithelin-mediated prevention of cell apoptosis. Moreover, the inhibition of the phosphatidylinositol 3-kinase and Akt abolished the effects of proepithelin on NF-?B activation. Finally, using siRNA and antisense strategies, we demonstrated that endogenously produced proepithelin by chondrocytes is important for chondrocyte growth in serum-deprived conditions. These results support the hypothesis that the induction of NF-?B activity of in growth plate chondrocytes is critical in proepithelin-mediated growth plate chondrogenesis and longitudinal bone growth.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Ccnd1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Grn protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/Rela protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor RelA,
http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1083-351X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
8
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| pubmed:volume |
286
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
24057-67
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| pubmed:meshHeading |
pubmed-meshheading:21566130-Animals,
pubmed-meshheading:21566130-Antioxidants,
pubmed-meshheading:21566130-Cell Differentiation,
pubmed-meshheading:21566130-Cell Proliferation,
pubmed-meshheading:21566130-Cells, Cultured,
pubmed-meshheading:21566130-Chondrocytes,
pubmed-meshheading:21566130-Chondrogenesis,
pubmed-meshheading:21566130-Cyclin D1,
pubmed-meshheading:21566130-Growth Plate,
pubmed-meshheading:21566130-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:21566130-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:21566130-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:21566130-Pyrrolidines,
pubmed-meshheading:21566130-Rats,
pubmed-meshheading:21566130-Rats, Sprague-Dawley,
pubmed-meshheading:21566130-Thiocarbamates,
pubmed-meshheading:21566130-Transcription Factor RelA
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| pubmed:year |
2011
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| pubmed:articleTitle |
Proepithelin stimulates growth plate chondrogenesis via nuclear factor-kappaB-p65-dependent mechanisms.
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| pubmed:affiliation |
First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Medical School of Xi'an Jiaotong University, Xi'an, 710061 China. shufangw@hotmail.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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