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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1990-4-25
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pubmed:databankReference |
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pubmed:abstractText |
We have characterized a retrotransposon in Trypanosoma brucei gambiense uniquely associated with the spliced-leader (SL) RNA gene cluster (Spliced Leader Associated Conserved Sequence, SLACS). There are nine copies of SLACS and DNA sequence analysis of one shows the hallmarks of Line-1 like elements. SLACS has generated a 49 bp target DNA duplication at its insertion site and its 3'-end is preceded by a poly(A) stretch. Two putative open reading frames (ORFs) span 75% of the element. ORF1 has CysHis motif associated with the retroviral gag polypeptide while ORF2 shows homology with reverse transcriptase sequences. Its 5'-end contains a repeated segment of a 185 bp that varies in copy number in different SLACS insertions. Retrotransposon-like sequences inserted into the SL-RNA genes occur in several hemoflagellates. These elements may represent a related family which has maintained its target site specificity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2408886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2418414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2421409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2430722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2439905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2441397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2445994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2447482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2464735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2552899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2827116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2828038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2842063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-2844414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-3023821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-3037321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-3137527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-3656436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-3692483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-3991809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6088060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6188156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6195530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6269745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6280868,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2156231-6327058
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
785-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2156231-Amino Acid Sequence,
pubmed-meshheading:2156231-Animals,
pubmed-meshheading:2156231-Base Sequence,
pubmed-meshheading:2156231-DNA, Ribosomal,
pubmed-meshheading:2156231-DNA Transposable Elements,
pubmed-meshheading:2156231-Mammals,
pubmed-meshheading:2156231-Molecular Sequence Data,
pubmed-meshheading:2156231-Molecular Weight,
pubmed-meshheading:2156231-Multigene Family,
pubmed-meshheading:2156231-RNA, Messenger,
pubmed-meshheading:2156231-RNA Splicing,
pubmed-meshheading:2156231-Restriction Mapping,
pubmed-meshheading:2156231-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2156231-Trypanosoma brucei gambiense
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pubmed:year |
1990
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pubmed:articleTitle |
SLACS retrotransposon from Trypanosoma brucei gambiense is similar to mammalian LINEs.
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pubmed:affiliation |
Yale MacArthur Center for Molecular Parasitology, New Haven, CT 06510.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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