| pubmed-article:2156216 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2156216 | lifeskim:mentions | umls-concept:C0001675 | lld:lifeskim |
| pubmed-article:2156216 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:2156216 | lifeskim:mentions | umls-concept:C1521970 | lld:lifeskim |
| pubmed-article:2156216 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:2156216 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:2156216 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:2156216 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2156216 | pubmed:dateCreated | 1990-4-13 | lld:pubmed |
| pubmed-article:2156216 | pubmed:abstractText | The patch-clamp technique was used to study Na+ channels of human skeletal muscle. Preparations were from biopsies of quadriceps muscle from adults who were not suffering from neuromuscular diseases. Activity of Na+ channels was recorded from inside-out patches when the membrane potential was stepped from a holding potential of -110 mV to potential above a threshold of about -65 mV. Single channel activity increased within minutes after hyperpolarizing the patch due to recovery from ultra-slow inactivation. Up to ten Na+ channels were active in individual patches. Macroscopic currents were reconstructed by averaging single channel currents. The time-to-peak current declined from 1.6 ms at -60 mV to 0.5 ms at + 10 mV. The currents decayed mono-exponentially with time constants between 12.1 ms at -60 mV and 0.4 ms at + 10 mV (21 C). The conductance of single Na+ channels was 1.65 pS and the mean open time was voltage-dependent. At -50 mV, the mean open time was 0.4 ms, while positive to -10 mV it increased to values above 1 ms. In the threshold potential range, the number of openings per depolarizing pulse was larger than the number of channels under the patch-clamp pipette, indicating reopening of Na+ channels at this potential. Openings could be observed only rarely 10 ms after onset of depolarization and the macroscopic current produced by late openings was less than 0.1% of the peak current. Human skeletal muscle is thus suitable for investigation with the patch-clamp technique and the determination of properties of Na+ channels with this technique could be the basis for an assessment of possible defects of these channels in diseased muscle. | lld:pubmed |
| pubmed-article:2156216 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2156216 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2156216 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2156216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2156216 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2156216 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2156216 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:2156216 | pubmed:author | pubmed-author:HattHH | lld:pubmed |
| pubmed-article:2156216 | pubmed:author | pubmed-author:FranklJJ | lld:pubmed |
| pubmed-article:2156216 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2156216 | pubmed:volume | 415 | lld:pubmed |
| pubmed-article:2156216 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2156216 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2156216 | pubmed:pagination | 399-406 | lld:pubmed |
| pubmed-article:2156216 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2156216 | pubmed:meshHeading | pubmed-meshheading:2156216-... | lld:pubmed |
| pubmed-article:2156216 | pubmed:meshHeading | pubmed-meshheading:2156216-... | lld:pubmed |
| pubmed-article:2156216 | pubmed:meshHeading | pubmed-meshheading:2156216-... | lld:pubmed |
| pubmed-article:2156216 | pubmed:meshHeading | pubmed-meshheading:2156216-... | lld:pubmed |
| pubmed-article:2156216 | pubmed:meshHeading | pubmed-meshheading:2156216-... | lld:pubmed |
| pubmed-article:2156216 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2156216 | pubmed:articleTitle | Characteristics of single Na+ channels of adult human skeletal muscle. | lld:pubmed |
| pubmed-article:2156216 | pubmed:affiliation | Physiologisches Institut der Technischen Universität München, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:2156216 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2156216 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2156216 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2156216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2156216 | lld:pubmed |
