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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1990-4-13
|
| pubmed:abstractText |
The patch-clamp technique was used to study Na+ channels of human skeletal muscle. Preparations were from biopsies of quadriceps muscle from adults who were not suffering from neuromuscular diseases. Activity of Na+ channels was recorded from inside-out patches when the membrane potential was stepped from a holding potential of -110 mV to potential above a threshold of about -65 mV. Single channel activity increased within minutes after hyperpolarizing the patch due to recovery from ultra-slow inactivation. Up to ten Na+ channels were active in individual patches. Macroscopic currents were reconstructed by averaging single channel currents. The time-to-peak current declined from 1.6 ms at -60 mV to 0.5 ms at + 10 mV. The currents decayed mono-exponentially with time constants between 12.1 ms at -60 mV and 0.4 ms at + 10 mV (21 C). The conductance of single Na+ channels was 1.65 pS and the mean open time was voltage-dependent. At -50 mV, the mean open time was 0.4 ms, while positive to -10 mV it increased to values above 1 ms. In the threshold potential range, the number of openings per depolarizing pulse was larger than the number of channels under the patch-clamp pipette, indicating reopening of Na+ channels at this potential. Openings could be observed only rarely 10 ms after onset of depolarization and the macroscopic current produced by late openings was less than 0.1% of the peak current. Human skeletal muscle is thus suitable for investigation with the patch-clamp technique and the determination of properties of Na+ channels with this technique could be the basis for an assessment of possible defects of these channels in diseased muscle.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
415
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
399-406
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1990
|
| pubmed:articleTitle |
Characteristics of single Na+ channels of adult human skeletal muscle.
|
| pubmed:affiliation |
Physiologisches Institut der Technischen Universität München, Federal Republic of Germany.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|