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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
2011-7-1
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pubmed:abstractText |
Ontogenesis of T cells in the thymus is a complex process whose molecular control is poorly understood. The present study investigated microRNAs involved in human thymocyte differentiation by comparing the microRNA expression profiles of thymocytes at the double-positive, single-positive CD4(+) and single-positive CD8(+) maturation stages. Microarray analysis showed that each thymocyte population displays a distinct microRNA expression profile that reflects their developmental relationships. Moreover, analysis of small-RNA libraries generated from human unsorted and double-positive thymocytes and from mature peripheral CD4(+) and CD8(+) T lymphocytes, together with the microarray data, indicated a trend toward up-regulation of microRNA expression during T-cell maturation after the double-positive stage and revealed a group of microRNAs regulated during normal T-cell development, including miR-150, which is strongly up-regulated as maturation progresses. We showed that miR-150 targets NOTCH3, a member of the Notch receptor family that plays important roles both in T-cell differentiation and leukemogenesis. Forced expression of miR-150 reduces NOTCH3 levels in T-cell lines and has adverse effects on their proliferation and survival. Overall, these findings suggest that control of the Notch pathway through miR-150 may have an important impact on T-cell development and physiology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1528-0020
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pubmed:author |
pubmed-author:AmadoriAlbertoA,
pubmed-author:BassoGiuseppeG,
pubmed-author:BassoKatiaK,
pubmed-author:BonannoLauraL,
pubmed-author:BronteVincenzoV,
pubmed-author:CorradinAlbertoA,
pubmed-author:D'AgostinoDonna MDM,
pubmed-author:De BellisGianlucaG,
pubmed-author:FrassonChiaraC,
pubmed-author:GerosaGinoG,
pubmed-author:GhisiMargheritaM,
pubmed-author:GuffantiAlessandroA,
pubmed-author:IndraccoloStefanoS,
pubmed-author:MukherjeeSubhamoyS,
pubmed-author:MussolinLaraL,
pubmed-author:RuggeroKatiaK,
pubmed-author:SerafinValentinaV,
pubmed-author:StellinGiovanniG,
pubmed-author:ZanovelloPaolaP
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pubmed:issnType |
Electronic
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pubmed:day |
30
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7053-62
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pubmed:meshHeading |
pubmed-meshheading:21551231-3' Untranslated Regions,
pubmed-meshheading:21551231-Adult,
pubmed-meshheading:21551231-Apoptosis,
pubmed-meshheading:21551231-Cell Differentiation,
pubmed-meshheading:21551231-Cell Line,
pubmed-meshheading:21551231-Cell Line, Tumor,
pubmed-meshheading:21551231-Cell Proliferation,
pubmed-meshheading:21551231-Cells, Cultured,
pubmed-meshheading:21551231-Child, Preschool,
pubmed-meshheading:21551231-Gene Expression Profiling,
pubmed-meshheading:21551231-Gene Expression Regulation,
pubmed-meshheading:21551231-Genes, Reporter,
pubmed-meshheading:21551231-Humans,
pubmed-meshheading:21551231-Infant,
pubmed-meshheading:21551231-Infant, Newborn,
pubmed-meshheading:21551231-MicroRNAs,
pubmed-meshheading:21551231-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:21551231-RNA, Messenger,
pubmed-meshheading:21551231-Receptors, Notch,
pubmed-meshheading:21551231-T-Lymphocyte Subsets,
pubmed-meshheading:21551231-T-Lymphocytes,
pubmed-meshheading:21551231-Thymus Gland
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pubmed:year |
2011
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pubmed:articleTitle |
Modulation of microRNA expression in human T-cell development: targeting of NOTCH3 by miR-150.
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pubmed:affiliation |
Department of Oncology and Surgical Sciences, University of Padova, Padova, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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